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January 2024
Home » January 2024 - Page 10 of 21 - IHC WORLD
January 2024
Decalcification Solutions for Bone
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Choose of decalcification solution will depend on the type of
Methods and Protocols for Decalcification of Bone Material
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Fixation: 10% Buffered Neutral Formalin for up to 5 days. All
Davidson’s Fixative Protocol
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Robert S. Richmond, M.D., F.C.A.P. Pathologist, USA Principle: Davidson's (sometimes called
Cryoprotectant Protocols for Storage of Free-Floating Brain Sections
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The following protocols are used for long term storage of
Clearing (Differentiation) Reagent for H&E Staining
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1% Acid Alcohol Solution:       Hydrochloric acid ---------------- 10 ml
Preparing a Cell Block from Cell Lines or Cell Suspensions
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Dr. Giorgio GattorettiAssociate Professor of Clinical PathologyInstitute for Cancer Genetics,
Bluing Reagents for H&E Staining
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Scotts Tap Water Substitute Scotts Tap Water Substitute is a bluing
Making and Using Aqueous Mounting Media
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Why buy a pre-mixed medium when it's cheap and easy
Immunofluorescence Double Staining Protocol – Squential Approach for Primary Antibodies Raised from Same Species
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1. Preparation of Slides  A. Cell Lines B. Frozen Sections C.
Immunofluorescence Double Staining Protocol – Parallel Approach
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1. Preparation of Slides  A. Cell Lines B. Frozen Sections C.
Immunohistochemistry Enzyme Double Staining Protocol
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1. Preparation of Slides  A. Cell Lines B. Frozen Sections C.
Double Immunofluorescence Staining for Bcl-6 and Else
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Dr. Giorgio Gattoretti Associate Professor of Clinical PathologyInstitute for Cancer
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