Reagents 0.1M Sodium Acetate Buffer, pH4.2: Methyl Green Solution (0.5%)

DAPI Counterstain Solution: DAPI is a popular nuclear counterstain for

PI Counterstain Solution: PI Stock Solution (1mg/ml or 1.5 mM):To

Nuclear Fast Red (Kernechtrot) Solution        Nuclear fast red ------------------------- 0.1

Chemicals  AmountFunction  Aluminum potassium sulfate (alum)50 gmordant Distilled water1000 mlsolventHematoxylin1 gdyeSodium iodate0.2 goxidizing

Chemicals  Single  Double  TripleFunction  Ethylene glycol  250 ml  250 ml  250 ml  solvent  Distilled water750 ml750 ml750 ml  solventHematoxylin2 g4