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Counterstain Protocols
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Counterstain Protocols
DAPI Fluorescence Counterstain Protocol
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DAPI Counterstain Solution: DAPI is a popular nuclear counterstain for
Propidium Iodide (PI) Fluorescence Counterstain Protocol
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PI Counterstain Solution: PI Stock Solution (1mg/ml or 1.5 mM):To
Nuclear Fast Red Counterstain Protocol
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Nuclear Fast Red (Kernechtrot) Solution        Nuclear fast red ------------------------- 0.1
Mayer’s Hematoxylin Protocol
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Chemicals  AmountFunction  Aluminum potassium sulfate (alum)50 gmordant Distilled water1000 mlsolventHematoxylin1 gdyeSodium iodate0.2 goxidizing
Gill’s Hematoxylin Protocol
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Chemicals  Single  Double  TripleFunction  Ethylene glycol  250 ml  250 ml  250 ml  solvent  Distilled water750 ml750 ml750 ml  solventHematoxylin2 g4