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Mayer’s Hematoxylin Protocol
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Mayer’s Hematoxylin Protocol
Chemicals  AmountFunction  
Aluminum potassium sulfate (alum)50 gmordant 
Distilled water1000 mlsolvent
Hematoxylin1 gdye
Sodium iodate0.2 goxidizing agent
Glacial acetic acid20 ml  pH control

Dissolve alum in distilled water. When alum is completely dissolved, add hematoxylin. When hematoxylin is completely dissolved, add sodium iodate and acetic acid. Bring to boil and cool. Filter if it is necessary.

Staining Characteristic


Suggested Use

Immunohistochemistry counterstain for 30 seconds.

H&E stain for 5 minutes.


This recipe gives clear and sharp nucleus staining with little background. Good for both immunohistochemistry counterstain and H&E staining. This solution is also alcohol and mercury free so it is good for IHC counterstain when AEC substrate is used.

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