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Bluing Reagents for H&E Staining
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Bluing Reagents for H&E Staining

0.1% Sodium Bicarbonate:

      Sodium bicarbonate --------------------------- 1 g

      Distilled water --------------------------------- 1000 ml

      Mix to dissolve. The pH will be around 8.0 Store this solution at room temperature.

      Note: this solution worked better than ammonia water solution according to testing result.

      Bluing for 30 seconds to 1 minute after hematoxylin staining and clearing/differentiation.

0.2% Ammonia Water Solution (Bluing):

      Ammonium hydroxide (concentrated) ------ 2 ml

      Distilled water -------------------------------- 1000 ml

      Mix well. The pH will be around 10.0 Store this solution at room temperature.

      Note: this solution is not as good as sodium bicarbonate according to testing result.

      Bluing for 30 seconds to 1 minute after hematoxylin staining and clearing/differentiation.

Lithium Carbonate Solution (Saturated):

      Lithium carbonate ---------------------------- 1.54 g

      Distilled water -------------------------------- 100 ml

      Mix to dissolve and store at room temperature.

      Bluing for 30 seconds to 1 minute after hematoxylin staining and clearing/differentiation.

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