Prepared by ROY ELLIS IMVS Division of Pathology The Queen

Solutions and Reagents: Eosin-Phloxine B Solution:       Eosin Stock Solution:       Eosin

Solutions and Reagents: 1% Acid Alcohol Solution (for differentiation):       Hydrochloric

Procedure Principal Factors for Good Sectioning of Frozen Specimens Recommended

Formalin Solution (10%, unbuffered):       Formaldehyde (37-40%) ------------- ----10 ml

Choose of decalcification solution will depend on the type of

Fixation: 10% Buffered Neutral Formalin for up to 5 days. All

Robert S. Richmond, M.D., F.C.A.P. Pathologist, USA Principle: Davidson's (sometimes called

The following protocols are used for long term storage of

1% Acid Alcohol Solution:       Hydrochloric acid ---------------- 10 ml

Dr. Giorgio GattorettiAssociate Professor of Clinical PathologyInstitute for Cancer Genetics,

Scotts Tap Water Substitute Scotts Tap Water Substitute is a bluing