Preparing Sections: Re-Fixation:     The following steps (step 4 through step

Using En Bloc Method to Increase Contrast Safety Note: Most

Fixation: Dehydration: Embedding: Embed in Beam capsules, and bake in

Fixation: 1.   Fix 1 mm tissue blocks in 4% formaldehyde and

Description: Tissues fixed in this solution followed by paraffin embedding and

J. A. Kiernan Department of Anatomy and Cell Biology The

Aqueous Mounting Medium (glycerol gelatin)  Kisser’sKaiser'sGlycerol jellyModifiedGelatine10 g40 g65 g5

Gelatin coated slides can hold sections on slides more securely

Prepared by ROY ELLIS IMVS Division of Pathology The Queen

Prepared by ROY ELLIS IMVS Division of Pathology The Queen