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January 2024
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January 2024 - Page 13 of 21 - IHC WORLD
January 2024
Tris-EDTA Buffer Antigen Retrieval Protocol
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Jan, 24
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Description: Formalin or other aldehyde fixation forms protein cross-links that mask
EDTA Buffer Antigen Retrieval Protocol
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21
Jan, 24
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Description: Formalin or other aldehyde fixation forms protein cross-links that mask
Citrate-EDTA Antigen Retrieval Protocol
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21
Jan, 24
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Description: Formalin or other aldehyde fixation forms protein cross-links that mask
Citrate Buffer Antigen Retrieval Protocol
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21
Jan, 24
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Description: Formalin or other aldehyde fixation forms protein cross-links that mask
Formic Acid Antigen Retrieval Protocol
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21
Jan, 24
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Description: Formalin or other aldehyde fixation forms protein cross-links that mask
DAPI Fluorescence Counterstain Protocol
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21
Jan, 24
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DAPI Counterstain Solution: DAPI is a popular nuclear counterstain for
Propidium Iodide (PI) Fluorescence Counterstain Protocol
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21
Jan, 24
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PI Counterstain Solution: PI Stock Solution (1mg/ml or 1.5 mM):To
Nuclear Fast Red Counterstain Protocol
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21
Jan, 24
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Nuclear Fast Red (Kernechtrot) Solution Nuclear fast red ------------------------- 0.1
Mayer’s Hematoxylin Protocol
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21
Jan, 24
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Chemicals AmountFunction Aluminum potassium sulfate (alum)50 gmordant Distilled water1000 mlsolventHematoxylin1 gdyeSodium iodate0.2 goxidizing
Gill’s Hematoxylin Protocol
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20
Jan, 24
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Chemicals Single Double TripleFunction Ethylene glycol 250 ml 250 ml 250 ml solvent Distilled water750 ml750 ml750 ml solventHematoxylin2 g4
Protocol for BCIP/NBT Alkaline Phosphatase Substrate Solution
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20
Jan, 24
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BCIP/NBT Alkaline Phosphatase Substrate Solution - Blue Final Dilution:
Protocol for New Fuchsin Alkaline Phosphatase Substrate Solution
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20
Jan, 24
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New Fuchsin Alkaline Phosphatase Substrate Solution – Red Final Dilution:
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