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Mayer’s Hematoxylin Protocol
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Chemicals  AmountFunction  Aluminum potassium sulfate (alum)50 gmordant Distilled water1000 mlsolventHematoxylin1 gdyeSodium iodate0.2 goxidizing
Gill’s Hematoxylin Protocol
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Chemicals  Single  Double  TripleFunction  Ethylene glycol  250 ml  250 ml  250 ml  solvent  Distilled water750 ml750 ml750 ml  solventHematoxylin2 g4
Protocol for BCIP/NBT Alkaline Phosphatase Substrate Solution
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BCIP/NBT Alkaline Phosphatase Substrate Solution - Blue   Final Dilution:        
Protocol for New Fuchsin Alkaline Phosphatase Substrate Solution
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New Fuchsin Alkaline Phosphatase Substrate Solution – Red   Final Dilution:        
Protocol for TMB Peroxidase Substrate Solution (Blue)
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TMB Peroxidase Substrate  Solution –  Blue Final Dilution:       0.005% TMB - 0.006% H2O2
Protocol for BDHC Peroxidase Substrate Solution (Blue)
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BDHC Peroxidase Substrate Solution –  Blue Final Dilution:        0.01% BDHC - 0.005% H2O2
Protocol for AEC Peroxidase Substrate Solution (Red)
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AEC (3-amino-9-ethylcarbazole) Peroxidase Substrate Solution – Red Final Dilution:        0.05% AEC
Protocol for DAB Peroxidase Substrate Solution (Blue)
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DAB Peroxidase Substrate Solution – Blue Final Dilution:        0.05% DAB,
Protocol for DAB Peroxidase Substrate Solution (Black)
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DAB Peroxidase Substrate Solution – Black Final Dilution:         0.05%
Protocol for DAB-Peroxidase Substrate Soluiton (Gray)
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DAB Peroxidase Substrate Solution - Gray Black Final Dilution:         0.05% DAB,
Protocol for DAB Peroxidase Substrate Solution (Brown)
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DAB Peroxidase Substrate Solution – Brown Final Dilution:       0.05% DAB
IHC Washing Buffer – AP Buffer
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AP Buffer (100mM Tris-HCl, 100mM NaCl, 5mM MgCl2, 0.05% Tween
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