Description: Tissues fixed in this solution followed by paraffin embedding and

J. A. Kiernan Department of Anatomy and Cell Biology The

Aqueous Mounting Medium (glycerol gelatin)  Kisser’sKaiser'sGlycerol jellyModifiedGelatine10 g40 g65 g5

Gelatin coated slides can hold sections on slides more securely

Prepared by ROY ELLIS IMVS Division of Pathology The Queen

Prepared by ROY ELLIS IMVS Division of Pathology The Queen

Solutions and Reagents: Eosin-Phloxine B Solution:       Eosin Stock Solution:       Eosin

Solutions and Reagents: 1% Acid Alcohol Solution (for differentiation):       Hydrochloric

Procedure Principal Factors for Good Sectioning of Frozen Specimens Recommended

Formalin Solution (10%, unbuffered):       Formaldehyde (37-40%) ------------- ----10 ml