Dr. Giorgio Cattoretti
Associate Professor of Clinical Pathology
Institute for Cancer Genetics, Columbia University
Immunohistochemistry (IHC) or immunofluorescence (IF)?
IHC allows you to see cytologic details and tissue architecture.
Preparations are permanent and relatively light-insensitive.
If you have very rare events, IHC allow you to screen large fields at low power.
Autofluorescence on some tissue makes IF very difficult.
IF gives quantitative signals (if you have a way to acquire signals quantitatively).
Co-staining the same subcellular structure with different fluorochrome is possible ONLY with IF.
Triple staining is possible only with IF.