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January 26, 2024
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January 26, 2024 - Page 6 of 7 - IHC WORLD
January 26, 2024
Electron Microscopy Negative Staining Protocol – Method 1
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26
Jan, 24
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Purpose: This protocol is used for staining virus, bacteria, microphage
TEM Immunogold Labeling Protocol – Post-embedding Method Using L.R. White Embedding Medium
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admin
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26
Jan, 24
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Procedure: 1. Fixation: fix specimen with 4% formaldehyde in 0.1M PB,
TEM Immunogold Labeling Protocol – Post-embedding Method Using EMBed812 as Embedding Medium
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admin
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26
Jan, 24
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Procedure: 1. Refer to routine TEM protocol as needed. 2. Sectioning: cut ultrathin
TEM Immunogold Labeling Protocol: Pre-embedding Method
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admin
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26
Jan, 24
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Procedure: 1. Sectioning: Vibratome sections at 50 um thick. 2. Collect sections in
Toluidine Blue Staining Protocol for Plastic Sections or Electron Microscopy (TEM) Thick (Semithin) Sections
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26
Jan, 24
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Description: This method is used for staining of TEM thick sections.
L.R. White Embedding Protocol for Electron Microscopy
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26
Jan, 24
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Overview: Resin embedding for LM provide greatly improved cellular definition
Routine TEM Processing and Embedding Protocol for Paraffin Sections Mounted on Slides
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26
Jan, 24
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Preparing Sections: Re-Fixation: The following steps (step 4 through step
Enhanced Transmission Electron Microscopy (TEM) Tissue Processing Technique
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admin
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26
Jan, 24
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Using En Bloc Method to Increase Contrast Safety Note: Most
Routine Transmission Electron Microscopy (TEM) Staining Protocol for Cultured Cells
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admin
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26
Jan, 24
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Fixation: Dehydration: Embedding: Embed in Beam capsules, and bake in
Routine Transmission Electron Microscopy (TEM) Staining Protocol for Tissues
By
admin
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26
Jan, 24
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Fixation: 1. Fix 1 mm tissue blocks in 4% formaldehyde and
Zinc Fixative (JB Fixative) Protocol
By
admin
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26
Jan, 24
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0 Comments
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Description: Tissues fixed in this solution followed by paraffin embedding and
Paraffin Section Method and Technique
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admin
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26
Jan, 24
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