Chemicals  AmountFunction  Aluminum potassium sulfate (alum)50 gmordant Distilled water1000 mlsolventHematoxylin1 gdyeSodium iodate0.2 goxidizing

Chemicals  Single  Double  TripleFunction  Ethylene glycol  250 ml  250 ml  250 ml  solvent  Distilled water750 ml750 ml750 ml  solventHematoxylin2 g4

BCIP/NBT Alkaline Phosphatase Substrate Solution - Blue   Final Dilution:        

New Fuchsin Alkaline Phosphatase Substrate Solution – Red   Final Dilution:        

TMB Peroxidase Substrate  Solution –  Blue Final Dilution:       0.005% TMB - 0.006% H2O2

BDHC Peroxidase Substrate Solution –  Blue Final Dilution:        0.01% BDHC - 0.005% H2O2

AEC (3-amino-9-ethylcarbazole) Peroxidase Substrate Solution – Red Final Dilution:        0.05% AEC

DAB Peroxidase Substrate Solution – Blue Final Dilution:        0.05% DAB,

DAB Peroxidase Substrate Solution – Black Final Dilution:         0.05%

DAB Peroxidase Substrate Solution - Gray Black Final Dilution:         0.05% DAB,

DAB Peroxidase Substrate Solution – Brown Final Dilution:       0.05% DAB

AP Buffer (100mM Tris-HCl, 100mM NaCl, 5mM MgCl2, 0.05% Tween