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January 2024
Protocol for TMB Peroxidase Substrate Solution (Blue)
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TMB Peroxidase Substrate  Solution –  Blue Final Dilution:       0.005% TMB - 0.006% H2O2
Protocol for BDHC Peroxidase Substrate Solution (Blue)
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BDHC Peroxidase Substrate Solution –  Blue Final Dilution:        0.01% BDHC - 0.005% H2O2
Protocol for AEC Peroxidase Substrate Solution (Red)
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AEC (3-amino-9-ethylcarbazole) Peroxidase Substrate Solution – Red Final Dilution:        0.05% AEC
Protocol for DAB Peroxidase Substrate Solution (Blue)
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DAB Peroxidase Substrate Solution – Blue Final Dilution:        0.05% DAB,
Protocol for DAB Peroxidase Substrate Solution (Black)
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DAB Peroxidase Substrate Solution – Black Final Dilution:         0.05%
Protocol for DAB-Peroxidase Substrate Soluiton (Gray)
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DAB Peroxidase Substrate Solution - Gray Black Final Dilution:         0.05% DAB,
Protocol for DAB Peroxidase Substrate Solution (Brown)
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DAB Peroxidase Substrate Solution – Brown Final Dilution:       0.05% DAB
IHC Washing Buffer – AP Buffer
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AP Buffer (100mM Tris-HCl, 100mM NaCl, 5mM MgCl2, 0.05% Tween
IHC Washing Buffer – Phosphate Buffer
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Phosphate Buffer (0.2M PB, pH 7.4):        Na2HPO4 (anhydrous) -------------------------- 21.8 g
IHC Washing Buffer – Tris-HCl Buffer
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10X Tris-HCl (0.5M Tris Base, pH7.6):       Trizma Base ---------------------------------- 61
IHC Washing Buffer- Tris Buffered Saline (TBS)
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10X TBS (0.5M Tris Base, 9% NaCl, pH 8.4):        Trizma
IHC Washing Buffer – Phosphate Buffered Saline (PBS)
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10X PBS (0.1M PBS, pH 7.4):        Na2HPO4 (anhydrous) ----------------------- 10.9
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