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Some DO’s and DON’T’s with Immunostaining of Cryostat Tissue Sections
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Chris van der Loos, PhDDepartment of PathologyAcademical Medical Center M2-230Meibergdreef…
How to Choose the Best Secondary Antibody for Immunohistochemistry Application
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Selection of the best secondary antibody can improve immunostaining and…
Strategies for Preventing Detachment of Sections From Glass Slides
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J. A. Kiernan Department of Anatomy and Cell Biology The…
Preventing Tissue Sections From Falling/Coming Off Slides
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Why my section falling (coming) off slide?How to prevent section…
Blocking Endogenous Biotin
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Why should I block endogenous biotin Some cells or tissues…
Blocking Endogenous Peroxidase
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Why should I block endogenous peroxidase acitivity Some cells or…
Antigen Retrieval Technical Tips
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What is antigen retrieval technique Tissue sections pretreated with the antigen…
Immunohistochemistry Troubleshooting
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Weak or No Staining SourcesSolutionsInadequate deparaffinizationDeparaffinize sections longer or change…
Methods and Techniques for Frozen Tissue Sections
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Procedure Principal Factors for Good Sectioning of Frozen Specimens Recommended…