|Why my section falling (coming) off slide?
|How to prevent section from falling off slide?
|Insufficient fixation or unfixed tissues tend to come off slides more easily.
|Fix tissues longer to get completely fixation of tissues.
|Formaldehyde fixed frozen sections are more prone to falling off slides.
|Try to dry slides for much longer time or use alternative fixation such as acetone or alcohol.
|Tissue sections tend to come off slides more often on regular slides (uncharged or uncoated).
|Always use positively charged or coated slides for immunostaining.
|You may just have had a bad batch of slides.
|Replace with a new batch of slides.
|Hand lotion or cream contaminating the waterbath.
|Avoid hand lotion or cream contaminating waterbath by wearing gloves.
|The disposable blades have oil on them.
|Clean disposable blades with xylene.
|Wrinkles presented in the sections during the initial mounting may contribute to problem for falling off sections.
|Try to spread the sections out and mount the sections on slides with wrinkle free.
|Paraffin sections may not be dried completely before placing in the oven.
|Allow paraffin sections to air dry at least 30 minutes before placing in the oven at 56 C overnight.
|Frozen sections may not be dried completely before fixation and immunostaining procedure.
|Allow frozen sections to air dry for at least 30 minutes before fixation and then air dry for another 30 minutes before immunostaining.
|Antigen retrieval procedure can cause sections to come off slides, especially when EDTA (pH8.0) or Tris-EDTA (pH9.0) such high pH antigen retrieval solution is used.
|Use low pH solution such as citrate buffer (pH6.0) antigen retrieval Solution to replace EDTA (pH8.0) or Tris-EDTA (pH9.0) retrieval solution if it is possible.
|Distilled water alone may make sections come off slides easy.
|Always use buffer solution to wash or rinse slides.
|Bone (especially the cartilage) tends to fall off slides after HIER.
|Try other alternative retrieval methods such as enzyme digestion.
|Antigen retrieval devices may be trouble.
|Try to use waterbath or steamer in stead of microwave or pressure cooker.
|Plain water in waterbath for mounting paraffin sections.
|Add some gelatin in waterbath for mounting paraffin sections.
|If you have tried everything above and the problem persists.
|Try gelatin coated slides and it has been working fairly well.