Protocol Database




Antigen Retrieval Protocols


The demonstration of many antigens can be significantly improved by the pretreatment with the antigen retrieval reagents that break the protein cross-links formed by formalin fixation and thereby uncover hidden antigenic sites.


Antigen Retrieval Overview

Room Temperature Epitope Retrieval (RTER)

Heat Induced Epitope Retrieval (HIER)

Proteolytic Induced Epitope Retrieval (PIER)

Frozen Section Epitope Retrieval

Free Floating Section Epitope Retrieval

Universal Antigen Retrieval Method

More antigen retrieval techniques and protocols