AP Buffer (100mM Tris-HCl, 100mM NaCl, 5mM MgCl2, 0.05% Tween

Phosphate Buffer (0.2M PB, pH 7.4):        Na2HPO4 (anhydrous) -------------------------- 21.8 g

10X Tris-HCl (0.5M Tris Base, pH7.6):       Trizma Base ---------------------------------- 61

10X TBS (0.5M Tris Base, 9% NaCl, pH 8.4):        Trizma

10X PBS (0.1M PBS, pH 7.4):        Na2HPO4 (anhydrous) ----------------------- 10.9