Bluing Reagents for H&E Staining

 

 

 

0.1% Sodium Bicarbonate:

 

      Sodium bicarbonate --------------------------- 1 g

      Distilled water --------------------------------- 1000 ml

      Mix to dissolve. The pH will be around 8.0 Store this solution at room temperature.

      Note: this solution worked better than ammonia water solution according to testing result.

      Bluing for 30 seconds to 1 minute after hematoxylin staining and clearing/differentiation.

 

0.2% Ammonia Water Solution (Bluing):

 

      Ammonium hydroxide (concentrated) ------ 2 ml

      Distilled water -------------------------------- 1000 ml

      Mix well. The pH will be around 10.0 Store this solution at room temperature.

      Note: this solution is not as good as sodium bicarbonate according to testing result.

      Bluing for 30 seconds to 1 minute after hematoxylin staining and clearing/differentiation.

 

Lithium Carbonate Solution (Saturated):

 

      Lithium carbonate ---------------------------- 1.54 g

      Distilled water -------------------------------- 100 ml

      Mix to dissolve and store at room temperature.

      Bluing for 30 seconds to 1 minute after hematoxylin staining and clearing/differentiation.