Klear Mouse Antibody on Mouse Tissue IHC Detection System

 

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Immunohistochemistry (IHC) Detection Systems

 

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Klear Mouse HRP Detection System

Polymer HRP detection system for

Mouse Antibody on Mouse Tissue

 


With GBI’s Klear Mouse Blocking reagent (Cat. No. D54-15) Mouse smooth muscle actin stained on mouse frozen liver tissue.


No Blocking. Mouse smooth muscle actin stained on mouse frozen liver tissue.

 

Features

  • For mouse antibody on mouse tissue

  • Biotin free system

  • Clean background and faster turnaround

  • Choose appropriate polymer-HRP for DAB and AEC

Klear Mouse HRP Detection System is used for visualizing mouse originated primary antibodies on mouse tissue section. Klear Mouse HRP detection system uses Polink-2 Plus technology to achieve ultra sensitive without a noticeable increase in background noise. The system is biotin-free and is provided in easy-to-use convenient dropper bottles.

The system contains block reagents that effectively block the secondary antibody from reacting with endogenous IgG on mouse tissue.

 

 

Catalog No.

Product Name

Mouse Blocking Buffer A&B

Chromogen

D52-110

Klear Mouse HRP for DAB bulk kit, 110 ml

Included

Not included

D52-15

Klear Mouse HRP with DAB kit 15ml

Included

DAB

D52-7

Klear Mouse HRP with DAB kit 7ml kit

Included

DAB

D53-110

Klear Mouse HRP for AEC Bulk kit, 110 ml

Included

Not included

D53-15

Klear Mouse HRP with AEC 15ml kit

Included

AEC

D53-7

Klear Mouse HRP with AEC 7ml kit

Included

AEC

D54-110

Klear Mouse Blocking A & B, 110 ml

Included

Not included

D54-15

Klear Mouse Blocking A & B, 15 ml

Included

Not included

 

 

Klear Mouse AP Detection System

Polymer AP detection system for

Mouse Antibody on Mouse Tissue

 

Features

  • For mouse antibody on mouse tissue with superior sensitivity and clean background

  • Require only 35 minutes of blocking compared to more than 1 hour blocking from other companies

  • Biotin free system. No biotin blocking is needed

Klear Mouse AP Detection System is used for visualizing mouse originated primary antibodies on mouse tissue section. Klear Mouse AP detection system uses Polink-2 Plus technology to achieve ultra sensitive without a noticeable increase in background staining. The system is biotin-free and is provided in easy-to-use convenient dropper bottles.

The system contains block reagents that effectively block the secondary antibody from reacting with endogenous IgG on mouse tissue.

 

Customer may choose Klear Mouse on Mouse AP with Fast Red or AP-Red+ according to the table.

 

 

Catalog No.

Product Name

Mouse Blocking Buffer A&B

Chromogen

D50-15

Klear Mouse on Mouse AP Fast Red kit, 15 ml

Included

Fast Red

D50-7

Klear Mouse on Mouse AP Fast Red kit, 7 ml

Included

Fast Red

D51-15

Klear Mouse on Mouse AP AP-Red+ kit, 15 ml

Included

AP-Red+

D51-7

Klear Mouse on Mouse AP AP-Red+ kit, 7 ml

Included

AP-Red+

 

 

Polymer-HRP and Polymer AP for Animal Tissue

Rat Antibody on Mouse Tissue

Mouse Antibody on Rat Tissue

 

Rat-on-Mouse Polymer Detection System

 

Detecting RAT primary antibody on MOUSE tissue is a very difficult task in research due to cross reactivity. Polink-1 HRP (or AP) RAT-NM (Non-Mouse) kits and Polink-2 HRP (or AP) RAT-NM kits are specially designed to solve this problem. This technology provides excellent specificity to detect rat primary antibody (user supplied) on mouse tissue. It is a biotin-free system, so therefore overcomes the non-specific staining caused by streptavidin/biotin system due to endogenous biotin. The secondary antibody was adsorbed to mouse, rabbit and human serum proteins. It also can be used on human and rabbit tissue. Specimen can be frozen or paraffin – embedded tissues, and freshly prepared monolayer cell smears.

 

One may choose Polink-1 HRP (or AP ) RAT-NM Detection kit for quicker turn around time. Polink-1 system is the ONE step polymer detection system that uses polymeric HRP-linked anti rat secondary antibody to directly detect rat primary antibody bound to the tissue. For ultra sensitivity, one may choose Polink-2 Plus HRP (or AP) Rat-NM kit. Choose appropriate polymer-HRP with DAB or AEC stain according to the table. Chromogen is not provided in Polink-2 AP Plus Rat kit. Refer to page 28-29 to choose preferred chromogen.

 

 

Catalog No.

Product Name

Primary Antibody

Chromogen

D35-110

Polink-1 HRP for DAB Rat-NM 110ml bulk

Rat

DAB

D35-15

Polink-1 HRP RAT-NM with DAB, 15 ml

Rat

DAB

D35-6

Polink-1 HRP RAT-NM with DAB, 6 ml

Rat

DAB

D36-110

Polink-1 HRP Rat-NM bulk for AEC, 110 ml

Rat

AEC

D36-15

Polink-1 HRP RAT-NM with AEC, 15 ml

Rat

AEC

D36-6

Polink-1 HRP RAT-NM with AEC, 6 ml

Rat

AEC

D62-110

Polink-1 AP Rat-NM bulk kit, 110 ml

Rat

Not included

D62-15

Polink-1 AP RAT-NM 15ml kit

Rat

Not included

D62-6

Polink-1 AP RAT-NM 6ml kit

Rat

Not included

D46-110

Polink-2 Plus HRP RAT-NM for DAB bulk Kit, 110 ml

Rat

DAB

D46-15

Polink-2 Plus HRP RAT-NM with DAB, 15 ml

Rat

DAB

D46-6

Polink-2 Plus HRP RAT-NM with DAB, 6 ml

Rat

DAB

D48-110

Polink-2 Plus HRP RAT-NM for AEC bulk Kit, 110 ml

Rat

AEC

D48-15

Polink-2 Plus HRP RAT-NM with AEC, 15 ml

Rat

AEC

D48-6

Polink-2 Plus HRP RAT-NM with AEC, 6 ml

Rat

AEC

D67-110

Polink-2 Plus AP RAT-NM Bulk Kit, 110 ml

Rat

Not included

D67-15

Polink-2 Plus AP RAT-NM 15ml kit

Rat

Not included

D67-6

Polink-2 Plus AP RAT-NM 6ml kit

Rat

Not included

 

Mouse-on-Rat Polymer Detection System

 

Detecting MOUSE primary antibody on RAT tissue is a very difficult task in research due to cross reactivity. Polink-1 HRP (or AP) MOUSE-NR (Non-Rat) kits and Polink-2 HRP (or AP) MOUSE-NR kits are specially designed to solve the problem. This technology provides excellent specificity to detect mouse primary antibody (user supplied) on rat tissue. It is a biotin-free system, so therefore overcomes the non-specific staining caused by streptavidin/biotin system due to endogenous biotin. The secondary antibody was adsorbed to rat, rabbit and human serum proteins. It also can be used on human and rabbit tissue. Specimen can be frozen or paraffin – embedded tissues, and freshly prepared monolayer cell smears.

 

One may choose Polink-1 HRP (or AP ) MOUSE-NR Detection kit for quicker turn around time. Polink-1 system is the ONE step polymer detection system that uses polymeric HRP-linked anti mouse secondary antibody to directly detect mouse primary antibody bound to the tissue. For ultra sensitivity, one may choose Polink-2 Plus HRP (or AP) MOUSE-NR kit. Choose appropriate polymer-HRP with DAB or AEC stain according to the table. Chromogen is not provided in Polink-2 Plus AP MOUSE-NR kit. Refer to page 28 to choose preferred chromogen.

 

 

Catalog No.

Product Name

Primary Antibody

Chromogen

D55-110

Polink-1 HRP for DAB Mouse-NR 110ml bulk

Mouse

DAB

D55-15

Polink-1 HRP MOUSE-NR with DAB, 15 ml

Mouse

DAB

D55-6

Polink-1 HRP MOUSE-NR with DAB, 6 ml

Mouse

DAB

D56-110

Polink-1 HRP Mouse- NR for AEC bulk, 110 ml

Mouse

AEC

D56-15

Polink-1 HRP MOUSE-NR with AEC, 15 ml

Mouse

AEC

D56-6

Polink-1 HRP MOUSE-NR with AEC, 6 ml

Mouse

AEC

D57-110

Polink-1 AP Mouse- NR bulk kit, 110 ml

Mouse

Not included

D57-15

Polink-1 AP MOUSE-NR 15ml kit

Mouse

Not included

D57-6

Polink-1 AP MOUSE-NR 6ml kit

Mouse

Not included

D58-110

Polink-2 Plus HRP MOUSE-NR for DAB Bulk Kit, 110 ml

Mouse

DAB

D58-15

Polink-2 Plus HRP MOUSE-NR with DAB, 15 ml

Mouse

DAB

D58-6

Polink-2 Plus HRP MOUSE-NR with DAB, 6 ml

Mouse

DAB

D59-110

Polink-2 Plus HRP MOUSE-NR for AEC Bulk Kit, 110 ml

Mouse

AEC

D59-15

Polink-2 Plus HRP MOUSE-NR with AEC, 15 ml

Mouse

AEC

D59-6

Polink-2 Plus HRP MOUSE-NR with AEC, 6 ml

Mouse

AEC

D65-110

Polink-2 Plus AP MOUSE-NR Bulk Kit, 110 ml

Mouse

Not included

D65-15

Polink-2 Plus AP MOUSE-NR 15ml kit

Mouse

Not included

D65-6

Polink-2 Plus AP MOUSE-NR 6ml kit

Mouse

Not included


 

How to Order


 

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