10X Tris-HCl (0.5M Tris Base, pH7.6):

      Trizma Base ---------------------------------- 61 g

      Distilled water ------------------------------- 1000 ml

      Adjust pH 7.6 using concentrated HCl

       Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.

20X Tris-HCl (1M Tris Base, pH7.6):

      Trizma Base ---------------------------------- 122 g

      Distilled water ------------------------------- 1000 ml

      Adjust pH 7.6 using concentrated HCl

      Store this solution at room temperature. Dilute 1:20 with distilled water before use and adjust pH if necessary.

10X Tris-HCl-Tween 20 (0.5M Tris Base, 0.5% Tween 20, pH7.6):

      Trizma Base ---------------------------------- 61 g

      Distilled water ------------------------------- 1000 ml

      Adjust pH 7.6 using concentrated HCl and then add 5 ml of Tween 20.

       Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.

Note: Tris-HCl Buffer is used for specific cases of immunohistochemical staining.