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Protocols
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Protocols
TEM Immunogold Labeling Protocol – Post-embedding Method Using L.R. White Embedding Medium
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Procedure: 1.   Fixation: fix specimen with 4% formaldehyde in 0.1M PB,
TEM Immunogold Labeling Protocol – Post-embedding Method Using EMBed812 as Embedding Medium
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Procedure: 1.   Refer to routine TEM protocol as needed.  2.   Sectioning: cut ultrathin
TEM Immunogold Labeling Protocol: Pre-embedding Method
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Procedure: 1.   Sectioning: Vibratome sections at 50 um thick. 2.   Collect sections in
Toluidine Blue Staining Protocol for Plastic Sections or Electron Microscopy (TEM) Thick (Semithin) Sections
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Description: This method is used for staining of TEM thick sections.
L.R. White Embedding Protocol for Electron Microscopy
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Overview: Resin embedding for LM provide greatly improved cellular definition
Routine TEM Processing and Embedding Protocol for Paraffin Sections Mounted on Slides
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Preparing Sections: Re-Fixation:     The following steps (step 4 through step
Enhanced Transmission Electron Microscopy (TEM) Tissue Processing Technique
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Using En Bloc Method to Increase Contrast Safety Note: Most
Routine Transmission Electron Microscopy (TEM) Staining Protocol for Cultured Cells
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Fixation: Dehydration: Embedding: Embed in Beam capsules, and bake in
Routine Transmission Electron Microscopy (TEM) Staining Protocol for Tissues
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Fixation: 1.   Fix 1 mm tissue blocks in 4% formaldehyde and
Zinc Fixative (JB Fixative) Protocol
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Description: Tissues fixed in this solution followed by paraffin embedding and
Coating Slides with a Film of Nitrocellulose
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J. A. Kiernan Department of Anatomy and Cell Biology The
Aqueous Mounting Medium Protocols
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Aqueous Mounting Medium (glycerol gelatin)  Kisser’sKaiser'sGlycerol jellyModifiedGelatine10 g40 g65 g5
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