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Protocols
IHC Washing Buffer – Tris-HCl Buffer
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10X Tris-HCl (0.5M Tris Base, pH7.6):       Trizma Base ---------------------------------- 61
IHC Washing Buffer- Tris Buffered Saline (TBS)
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10X TBS (0.5M Tris Base, 9% NaCl, pH 8.4):        Trizma
IHC Washing Buffer – Phosphate Buffered Saline (PBS)
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10X PBS (0.1M PBS, pH 7.4):        Na2HPO4 (anhydrous) ----------------------- 10.9
Antibody Dilution Buffers
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Antibody dilution buffer is used to dilute primary and secondary
Blocking Solutions
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Background or unspecific staining often occurs for IHC antibody staining.
Hydrochloric Acid (HCl) Antigen Retrieval Protocol
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Description: Formalin or other aldehyde fixation forms protein cross-links that mask
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