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Protocols
Oil Red O Staining Protocol (Ellis)
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Prepared by ROY ELLIS IMVS Division of Pathology The Queen
Oil Red O Staining Protocol
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Description: This protocol is for lipid and fat staining on frozen
Nissl Staining Method and Protocol on Frozen or Vibratome Sections for Brain & Spinal Cord
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Description: This method is used for the detection of Nissl body
Nissl Staining Method and Protocol on Paraffin Sections for Brain & Spinal Cord
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Description: This method is used for the detection of Nissl body
Miller’s Elastic Staining Protocol
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Prepared by ROY ELLIS IMVS Division of Pathology The Queen
Microglia Staining Protocol
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Description: This method is for demonstration of microglia in brain tissue.
Methenamine Silver (Gomori PAMS) Staining Protocol for Reticular Fibers and Basement Membranes
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Solutions and Reagents: 0.5% Aqueous Periodic Acid Methenamine Silver Stock
Masson’s Trichrome Staining Protocol for Collagen Fibers
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Description: This method is used for the detection of collagen fibers
Masson Fontana Staining Protocol
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Prepared by ROY ELLIS IMVS Division of Pathology The Queen
Luxol Fast Blue Staining Protocol for Myelin
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Description: This is for staining of myelin/myelinated axons on formalin-fixed, paraffin-embedded
Luna Staining Method and Protocol for Elastic Fibers and Mast Cells
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Description: This method is used for identifying elastic fibers and mast
Hyaluronidase Alcian Blue Staining Protocol
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Prepared by ROY ELLIS IMVS Division of Pathology The Queen
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