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IHC Staining
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IHC Staining
ISH decal specimens
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Question. Recently, the Kappa/Lambda ISH has quit working on our…
Shelf life of working antibody solutions in IHC
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Question. I have tried to find a general instruction for…
Maximum thickness of brain sections in slide for IHC staining
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Question. What is the maximum thickness that I could use…
IF staining questions
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Question. Why would one decide to use a primary antibody…
DAB freezing
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Question. I am doing free-floating immunohistochemistry with brain slices. The…
Do I need to freeze my antibody aliquots?
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Dr. Giorgio CattorettiAssociate Professor of Clinical PathologyInstitute for Cancer Genetics,…
How stable are the diluted antibodies?
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Dr. Giorgio CattorettiAssociate Professor of Clinical PathologyInstitute for Cancer Genetics,…
For how long should I incubate a primary antibody?
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Dr. Giorgio CattorettiAssociate Professor of Clinical PathologyInstitute for Cancer Genetics,…
Do I need detergent to permeabilize the cells?
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Dr. Giorgio CattorettiAssociate Professor of Clinical PathologyInstitute for Cancer Genetics,…
Which is the most sensitive, HRP or AP?
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Dr. Giorgio CattorettiAssociate Professor of Clinical PathologyInstitute for Cancer Genetics,…
Alkaline Phospatase (AP) or Peroxidase (HRP)?
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Dr. Giorgio CattorettiAssociate Professor of Clinical PathologyInstitute for Cancer Genetics,…
Which is the most sensitive method? IF or IHC?
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Dr. Giorgio CattorettiAssociate Professor of Clinical PathologyInstitute for Cancer Genetics,…