Chemicals  Single  Double  TripleFunction  Ethylene glycol  250 ml  250 ml  250 ml  solvent  Distilled water750 ml750 ml750 ml  solventHematoxylin2 g4

BCIP/NBT Alkaline Phosphatase Substrate Solution - Blue   Final Dilution:        

New Fuchsin Alkaline Phosphatase Substrate Solution – Red   Final Dilution:        

TMB Peroxidase Substrate  Solution –  Blue Final Dilution:       0.005% TMB - 0.006% H2O2

BDHC Peroxidase Substrate Solution –  Blue Final Dilution:        0.01% BDHC - 0.005% H2O2

AEC (3-amino-9-ethylcarbazole) Peroxidase Substrate Solution – Red Final Dilution:        0.05% AEC

DAB Peroxidase Substrate Solution – Blue Final Dilution:        0.05% DAB,

DAB Peroxidase Substrate Solution – Black Final Dilution:         0.05%

DAB Peroxidase Substrate Solution - Gray Black Final Dilution:         0.05% DAB,

DAB Peroxidase Substrate Solution – Brown Final Dilution:       0.05% DAB

AP Buffer (100mM Tris-HCl, 100mM NaCl, 5mM MgCl2, 0.05% Tween

Phosphate Buffer (0.2M PB, pH 7.4):        Na2HPO4 (anhydrous) -------------------------- 21.8 g