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Immunohistochemistry (IHC) Detection Systems
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Polink-2 Plus IHC Detection System
New Generation of 2-step Polymer Detection System
for Immunohistochemistry
Features
Current 2-step polymer-HRP and polymer-AP detection system are widely accepted in the field of immunohistochemistry. However, the current 2-step polymer detection system demonstrate 3 weaknesses: 1. It can only enhance the signal for mouse primary antibodies and does not enhance the signal for rabbit primary antibodies. 2. The current 2-step polymer detection system offers customers only for broad spectrum (for mouse and rabbit) due to the limitation of the bridging antibody. 3. The current 2-step polymer system does not provide satisfactory sensitivity when detecting rabbit antibodies, goat antibodies and rat antibodies. Through new conjugation technology, Polink-2 Plus Series overcomes the weaknesses of current 2-step polymer detection systems. It demonstrates ultra sensitivity and high specificity to mouse, rabbit, goat and rat antibodies. This system reduces incubation time by 10 minutes for each incubation step, saving a total of 20-30 minutes for the entire process.
Polink-2 Plus Polymer HRP or AP Detection Kits for
Broad Spectrum (Mouse & Rabbit Primary Antibody)
Polink-2 Plus HRP DAB Broad kit stained mouse anti-ER on human breast cancer tissue.
Polink-2 Plus Detection Kits for broad spectrum (mouse and rabbit) demonstrate excellent sensitivity to mouse and rabbit antibody with choice of Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP) conjugate. This innovative technology surpasses other 2-step polymer technologies when detecting rabbit antibodies. It’s super sensitivity allows customers to detect difficult-to-work antibodies.
Polink-2 Plus HRP
Broad kit
stained rabbit anti glucagon 1:400 on human pancreas.
|
A leading
competitor’s 2-step polymer kitstained rabbit anti glucagon 1:400 on
humanpancreas.
|
Catalog No. |
Product Name |
Serum Adsorbed |
Chromogen |
D41-110 |
Polink-2 Plus HRP Broad for DAB Bulk kit, 110 ml |
Human |
Not included |
D41-15 |
Polink-2 Plus HRP Broad with DAB Kit, 15 ml |
Human |
DAB |
D41-6 |
Polink-2 Plus HRP Broad with DAB Kit, 6 ml |
Human |
DAB |
D42-110 |
Polink-2 Plus HRP Broad for AEC Bulk kit, 110 ml |
Human |
Not included |
D42-15 |
Polink-2 Plus HRP Broad with AEC kit, 15 ml |
Human |
AEC |
D42-6 |
Polink-2 Plus HRP Broad with AEC kit, 6 ml |
Human |
AEC |
D68-110 |
Polink-2 Plus AP Broad Bulk kit, 110 ml |
Human |
Not included |
D68-15 |
Polink-2 Plus AP Broad kit, 15 ml |
Human |
Not included |
D68-6 |
Polink-2 Plus AP Broad kit, 6 ml |
Human |
Not included |
Polink-2 Plus Polymer HRP or AP Detection Kits for
Mouse Primary antibody
Mouse anti B Cell 1:400 stained Tonsil by Polink-2 Plus Mouse DAB Kit (Cat. No. D37-15)
Polink-2 Plus Detection Mouse Kits demonstrate excellent sensitivity to mouse antibody with choice of Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP) conjugate. This innovative technology surpasses other polymer technologies by 2-4 fold when detecting difficult-to-work antibodies.
Mouse anti ER 1:400 by Polink-2 Plus HRP Mouse DAB Kit (Cat. No. D37-15) |
Mouse anti ER 1:100 by a leading company’s polymer detection system |
Catalog No. |
Product Name |
Serum Adsorbed |
Chromogen |
D37-110
|
Polink-2 Plus HRP MOUSE for DAB Bulk Kit, 110 ml |
Human, Rabbit |
Not included |
D37-15
|
Polink-2 Plus HRP MOUSE with DAB kit, 15 ml |
Human, Rabbit |
DAB |
D37-6
|
Polink-2 Plus HRP MOUSE with DAB kit, 6 ml |
Human, Rabbit |
DAB |
D38-110
|
Polink-2 Plus HRP MOUSE for AEC Bulk kit, 110 ml |
Human, Rabbit |
Not included |
D38-15
|
Polink-2 Plus HRP MOUSE with AEC Kit, 15 ml |
Human, Rabbit |
AEC |
D38-6
|
Polink-2 Plus HRP MOUSE with AEC Kit, 6 ml |
Human, Rabbit |
AEC |
D69-110
|
Polink-2 Plus AP MOUSE Bulk kit, 110 ml |
Human, Rabbit |
Not included |
D69-15
|
Polink-2 Plus AP MOUSE kit, 15 ml |
Human, Rabbit |
Not included |
D69-6
|
Polink-2 Plus AP MOUSE kit, 6 ml |
Human, Rabbit |
Not included |
Polink-2 Plus Polymer HRP or AP Detection Kits for
Rabbit Primary Antibody
Polink-2 Plus HRP or AP Rabbit Detection Kit is an ultra sensitive polymer detection system. It uses rabbit antibody specific enhancer to help amplify the polymer-enzyme conjugate reaction to achieve super sensitivity and specificity in immunohistochemistry staining. It produces 2-4 fold sensitivity compared to other 2-step polymer detection system on the market. User may need to further dilute primary antibody due to super sensitivity of Polink-2 Plus detection system. It is a biotin-free system, therefore, overcomes the non-specific staining caused by streptavidin /biotin system due to endogenous biotin. Most commonly used specimens for this system are: frozen tissue, paraffin-embedded tissue, freshly prepared lymphocytes and fixed culture cells. It is compatible either with manual or automated systems. Staining conditions need to be optimized by user.
Rabbit anti S-100 1:400 stained on human skin by Polink-2 Plus HRP DAB Rabbit Kit (Cat. D39-15) |
Rabbit anti S-100 1:400 stained on human skin by 2-step polymer HRP kit from a leading competitor |
Catalog No. |
Product Name |
Serum Adsorbed |
Chromogen |
D39-110 |
Polink-2 Plus HRP RABBIT for DAB Bulk Kit, 110 ml |
Human, Mouse |
Not included |
D39-15 |
Polink-2 Plus HRP RABBIT with DAB kit, 15 ml |
Human, Mouse |
DAB |
D39-6
|
Polink-2 Plus HRP RABBIT with DAB kit, 6 ml |
Human, Mouse |
DAB |
D40-110
|
Polink-2 Plus HRP RABBIT for AEC Bulk kit, 110 ml |
Human, Mouse |
Not included |
D40-15
|
Polink-2 Plus HRP RABBIT with AEC kit, 15 ml |
Human, Mouse |
AEC |
D40-6
|
Polink-2 Plus HRP RABBIT with AEC kit, 6 ml |
Human, Mouse |
AEC |
D70-110
|
Polink-2 Plus AP Rabbit Bulk kit, 110 ml |
Human, Mouse |
Not included |
D70-15
|
Polink-2 Plus AP Rabbit kit, 15 ml |
Human, Mouse |
Not included |
D70-6
|
Polink-2 Plus AP Rabbit kit, 6 ml |
Human, Mouse |
Not included |
Polink-2 Plus Polymer HRP or AP Detection Kits for
Goat Primary Antibody
Polink-2 Plus HRP or AP Goat Detection Kit is an ultra-sensitive polymer detection system. It uses Goat antibody specific enhancer to help amplify the polymer-enzyme conjugate reaction to achieve super sensitivity and specificity in immunohistochemistry staining. It produces consistent immunostaining outcomes on archival tissues and on difficult-to-work antibodies. User may need to further dilute primary antibody due to the super sensitivity of Polink-2 Plus detection system. It is a biotin-free system, therefore, overcomes the non-specific staining caused by streptavidin/biotin systems due to endogenous biotin. Most commonly used specimens for this system are: frozen tissue, paraffin-embedded tissue, freshly prepared lymphocytes and fixed culture cells. It is compatible with either manual or automated systems. Staining conditions need to be optimized by user.
Goat anti P53 1:200 stained on human breast cancer by Polink-2 Plus HRP Goat AEC Kit (Cat. No.D45-15) |
Goat anti P53 1:200 stained on human breast cancer by 1-step polymer HRP Goat AEC kit from a leading competitor |
Catalog No. |
Product Name |
Serum Adsorbed |
Chromogen |
D43-110
|
Polink-2 Plus HRP GOAT for DAB Bulk kit, 110 ml |
Human, Mouse, Rabbit |
Not included |
D43-15
|
Polink-2 Plus HRP GOAT with DAB kit, 15 ml |
Human, Mouse, Rabbit |
DAB |
D43-6 |
Polink-2 Plus HRP GOAT with DAB kit, 6 ml |
Human, Mouse, Rabbit |
DAB |
D45-110
|
Polink-2 Plus HRP GOAT for AEC Bulk kit, 110 ml |
Human, Mouse, Rabbit |
Not included |
D45-15
|
Polink-2 Plus HRP GOAT with AEC kit, 15 ml |
Human, Mouse, Rabbit |
AEC |
D45-6 |
Polink-2 Plus HRP GOAT with AEC kit, 6 ml |
Human, Mouse, Rabbit |
AEC |
D66-110
|
Polink-2 Plus AP GOAT Bulk kit, 110 ml |
Human, Mouse, Rabbit |
Not included |
D66-15
|
Polink-2 Plus AP GOAT kit, 15 ml |
Human, Mouse, Rabbit |
Not included |
D66-6 |
Polink-2 Plus AP GOAT kit, 6 ml |
Human, Mouse, Rabbit |
Not included |
Polink-2 Plus Polymer HRP or AP Detection Kits for
Rat
Primary antibodyDetecting RAT primary antibody on MOUSE tissue is a very difficult task in research field due to background staining issues. Polink-2 Plus HRP or AP Rat-NM Detection Kit is specially designed to solve the problem. The secondary antibody is adsorbed to mouse and human serum proteins. This technology provides excellent specificity to detect rat primary antibody (user supplied) on mouse tissue. Besides mouse tissue Polink-2 Plus Rat-NM Detection kit also can be used on human tissue as well.
Rat anti Podoplanin 1:800 stained on
human skin by Polink-2 Plus HRP Rat-NM DAB kit (Cat.
No. D46-15)
|
Rat anti Podoplanin 1:800 stained on
human skin by 1-step polymer HRP kit
|
Catalog No. |
Product Name |
Serum Adsorbed |
Chromogen |
D46-110 |
Polink-2 Plus HRP RAT-NM for DAB bulk Kit, 110 ml |
No cross react to mouse |
Not included |
D46-15
|
Polink-2 Plus HRP RAT-NM with DAB kit, 15 ml |
No cross react to mouse |
DAB |
D46-6 |
Polink-2 Plus HRP RAT-NM with DAB kit. 6 ml |
No cross react to mouse |
DAB |
D48-110
|
Polink-2 Plus HRP RAT-NM for AEC bulk Kit, 110 ml |
No cross react to mouse |
Not included |
D48-15
|
Polink-2 Plus HRP RAT-NM with AEC Kit, 15 ml |
No cross react to mouse |
AEC |
D48-6
|
Polink-2 Plus HRP RAT-NM with AEC Kit, 6 ml |
No cross react to mouse |
AEC |
D67-110
|
Polink-2 Plus AP RAT-NM Bulk Kit, 110 ml |
No cross react to mouse |
Not included |
D67-15
|
Polink-2 Plus AP RAT-NM kit, 15 ml |
No cross react to mouse |
Not included |
D67-6 |
Polink-2 Plus AP RAT-NM kit, 6 ml |
No cross react to mouse |
Not included |
Polink-2 Plus Polymer HRP or AP Detection Kits for
Mouse Primary antibody on Rat tissue
Detecting MOUSE primary antibody on RAT tissue is a very difficult task in research field due to background staining issues. Polink-2 Plus HRP or AP Mouse-NR (no cross react to rat) Detection Kit is specially designed to solve the problem. The secondary antibody is adsorbed to rat, rabbit and human serum proteins. This technology provides excellent specificity to detect mouse primary antibody (user supplied) on rat tissue. Besides rat tissue Polink-2 Plus Mouse-NR Detection kit also can be used on human tissue and rabbit tissue as well.
Polink-2 Plus Mouse-NR Detection Kit is a ultra sensitive polymer detection system. It uses mouse antibody enhancer to help amplify the polymer-enzyme conjugate reaction to achieve super sensitivity and specificity in immunohistochemistry staining. It produces consistent immunostaining outcomes on archival tissues and on difficult-to-work antibodies. User may need to further dilute primary antibody due to super sensitivity of Polink-2 Plus detection system. It is a biotin-free system, therefore it overcomes the non-specific staining caused by streptavidin/biotin system due to endogenous biotin. Most commonly used specimens for this system are: frozen tissue, paraffin-embedded tissue, freshly prepared lymphocytes and fixed culture cells. It is compatible with either manual or automated systems. Staining conditions need to be optimized by user.
Mouse anti S-100 on RAT skin stained by Polink-2 Plus AP Mouse-NR Kit (Cat.No. D65-15) |
Mouse anti S-100 on RAT brain stained by Polink-2 Plus AP Mouse-NR Kit (Cat.No. D65-15) |
Catalog No. |
Product Name |
Serum Adsorbed |
Chromogen |
D58-110
|
Polink-2 Plus HRP MOUSE-NR for DAB Bulk Kit, 110 ml |
No cross react to rat |
Not included |
D58-15
|
Polink-2 Plus HRP MOUSE-NR with DAB Kit, 15 ml |
No cross react to rat |
DAB |
D58-6 |
Polink-2 Plus HRP MOUSE-NR with DAB Kit, 6 ml |
No cross react to rat |
DAB |
D59-110
|
Polink-2 Plus HRP MOUSE-NR for AEC Bulk Kit, 110 ml |
No cross react to rat |
Not included |
D59-15
|
Polink-2 Plus HRP MOUSE-NR with AEC Kit, 15 ml |
No cross react to rat |
AEC |
D59-6 |
Polink-2 Plus HRP MOUSE-NR with AEC Kit, 6 ml |
No cross react to rat |
AEC |
D65-110
|
Polink-2 Plus AP MOUSE-NR Bulk Kit, 110 ml |
No cross react to rat |
Not included |
D65-15
|
Polink-2 Plus AP MOUSE-NR kit, 15 ml |
No cross react to rat |
Not included |
D65-6 |
Polink-2 Plus AP MOUSE-NR kit, 6 ml |
No cross react to rat |
Not included |
How to Order
PO can be Emailed to sales@ihcworld.com or Faxed to 443-638-0394
Credit Card Order need to call us at 443-686-9618
Online Order can be placed on our Online Store at http://store.ihcworld.com/
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