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Immunohistochemistry (IHC) Detection Systems

 

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Mounting Mediums

 

GB-Mount is a water-based mounting media for using with frozen section, immunoperoxidase and immunofluorescent procedures. AEC, AP-Red, and AP-Blue are soluble in alcohol and organic solvents. Tissue slides processed by these methods should be coverslipped with GB-Mount. Immunofluorescent assays require a mounting media that will not interfere with the assay results. When appropriate filters used, GB-Mount will not show any fluorescence background. Besides, there is no risk of quenching fluorescent emission. GB-Mount is supplied in a 15 ml a dropper bottle or 100ml bottle.

 

Simpo-Mount is water-based mounting medium for immunohistology. It is used for the permanent preservation of the following chromogens or dyes. Simply apply drops of Simpo-Mount on the tissue section a liquid polymer coverslip will form. It does not need coverslip. This universal mounting media maintains the original stain intensity and characteristics. It demonstrates excellent optical clarity.

 

ISH Mounting Medium is designed for permanent preservation of tissue sections and cell smears with alkaline phosphatase chromogens like NBT/BCIP and red stain. ISH Mount is supplied in 15 ml dropper bottle or 100ml bottle.

 

Fluorescent Mounting Medium is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photo bleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox, Phycoerythrin (RP-E), phyocyanin (PC), and allophycocyanin (APC). The fluorescence is retained during prolonged storage at 4 C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC.

 

Fluorescent Mounting Medium with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). Fluorescence is retained during prolonged storage at 4oC in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC. This mounting medium is fortified with DAPI which is a counter-stain for DNA. This product is to be used in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI.

 

Fluorescent Mounting Medium with PI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). Fluorescence is retained during prolonged storage at 4oC in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC. This mounting medium is fortified with PI which is a counter-stain for DNA. This product is to be used in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. PI excites at 535nm and emits at 615nm, producing a red fluorescence. RNA is also stained with PI.

 

Catalog No.

Product Name

Size

Aqueous/Organic

Suitable for

E01-15

GB-Mount

15 ml

Aqueous

AEC, AP-Red, AP-Blue

E03-100

Simpo-Mount

100 ml

Aqueous

DAB, AEC, BCIP/NBT, BCIP/INT, Fast-Red, AP-Red, FITC, Rhodamine, Texas Red, FluoroBlue

E03-15

Simpo-Mount

15 ml

Aqueous

DAB, AEC, BCIP/NBT, BCIP/INT, Fast-Red, AP-Red, FITC, Rhodamine, Texas Red, FluoroBlue

E21-100

ISH Mount Bulk kit

100 ml

 

BCIP/NBT or red stain

E18-100

Fluorescent Mounting Medium 

100 ml

Aqueous

FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 488, Alexa fluoro 594, GFP, tetramethyly rhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), Allophycocyanin (APC)

E18-15

Fluorescent Mounting Medium

15 ml

Aqueous

FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 488, Alexa fluoro 594, GFP, tetramethyly rhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), Allophycocyanin (APC)

E19-100

Fluorescent Mounting Medium with DAPI

100 ml

Aqueous

FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 488, Alexa fluoro 594, GFP, Tetramethyly Rhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), Allophycocyanin (APC)

E19-15

Fluorescent Mounting Medium with DAPI

15 ml

Aqueous

FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 488, Alexa fluoro 594, GFP, Tetramethyly Rhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), Allophycocyanin (APC)

E20-100

Fluorescent Mounting Medium with PI

100 ml

Aqueous

FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 488, Alexa fluoro 594, GFP, Tetramethyly Rhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), Allophycocyanin (APC)

E20-15

Fluorescent Mounting Medium with PI

15 ml

Aqueous

FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 488, Alexa fluoro 594, GFP, Tetramethyly Rhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), Allophycocyanin (APC)



 

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