NovaUltraTM
Special Stain Kits
Histology special stains use reagents and solutions made from histological dyes to identify and demonstrate specific structures and cellular components that aid pathologists in the evaluation of certain diseases. IHC World offers the most commonly used special stain kits for your research needs. All of our special stain kits contains solutions that are pre-diluted, ready to use form. A step by step protocol is provided for each kit for the convenience of users. IHC World also offers the biological dyes for customers wishing to prepare their own staining solutions.
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How to Order
PO can be Emailed to sales@ihcworld.com or Faxed to 443-638-0394
Credit Card Order need to call us at 443-686-9618
Online Order can be placed on our Online Store at http://store.ihcworld.com/
NovaUltraTM H&E Stain Kit | |
Special formulated hematoxylin and eosin solution for quality and fast H&E staining with no need to perform bluing nor clearing/differentiation steps. Nuclei are stained blue crispy clear color with pink cytoplasmic and background color.
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Unit: Kit Price: $195
The kit components:
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NovaUltraTM Alcian Blue Stain Kit | |
This kit is designed to stain formalin
fixed, paraffin embedded tissue sections, and may be used
for frozen sections as well. Strongly acidic mucosubstances
will be stained blue, nuclei will be stained pink to red,
and cytoplasm will be stained pale pink.
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Unit: Kit Price: $195
The kit components:
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NovaUltraTM Alcian Blue - PAS Stain Kit | |
This kit utilizes the properties of both the PAS and Alcian Blue methods to demonstrate the full complement of tissue proteoglycans. The rationale of the technique is that by first staining all the acidic mucins with Alcian Blue, those acidic mucins which are also PAS positive will be chemically blocked and will not react further during the technique. Those neutral mucins which are solely PAS positive will subsequently be demonstrated in a contrasting manner. The combined result demonstrates both acidic and neutral mucins as well as mixtures of acidic and neutral mucins. |
Unit: Kit Price: $295
The kit components:
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NovaUltraTM Alizarin Red Stain Kit | |
Alizarin Red S, an anthraquinone derivative, may be used to identify calcium in tissue sections. The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but these elements usually do not occur in sufficient concentration to interfere with the staining. Calcium forms an Alizarin Red S-calcium complex in a chelation process, and the end product is birefringent.
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Unit: Kit Price: $95
The kit components:
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NovaUltraTM Congo Red Stain Kit | |
This kit is a modified Highman's Congo Red stain. It is used
for the detection of amyloid on formalin-fixed,
paraffin-embedded tissue sections with amyloidosis, and may
be used for frozen sections as well. The amyloid deposits
will be stained red and the nuclei will be stained blue. The
thickness of sections is usually 5 um. But in case of
inadequate amyloid deposits, 10um thick sections will be
more satisfactory.
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Unit: Kit Price: $195
The kit components:
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NovaUltraTM Fontana Masson Stain Kit | |
Fontana-Masson stains argentaffin granules and melanin. Melanin is a nonlipid, nonhematogenous pigment. It is a brown-black pigment present normally in the hair, skin, retina, iris, and certain parts of CNS. Argentaffin granules are found in carcinoid tumors. This kit is designed to stain formalin fixed, paraffin embedded tissue sections, and may be use for frozen sections as well. The argentaffin cell granules or melanin will be stained black. The nuclei will be stained pink-red. The cytoplasm will be stained pale pink.
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Unit: Kit Price: $295
The kit components:
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NovaUltraTM Golgi-Cox Stain Kit | |
The Golgi-Cox Kit is designed for the staining of neuronal
dendrites and dendritic spines on fresh or paraformaldehyde
fixed brain tissues. The Kit has been extensively tested on
both fresh and paraformaldehyde fixed brain tissues in rats
and mice and should work on other species as well. The
impregnation will take 10-14 days depending on the age and
size of the tissues. The Kit only contains two ready-to-use
reagents and the protocol is easy to follow and the staining
quality is excellent.
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Unit: Kit Price: $295
The kit components:
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NovaUltraTM Hema-Diff Stain Kit | |
Three step procedure for differential staining of blood smears. Used to evaluate blood parasites, microbial organisms and bone marrow specimens, Hema-Diff™ is used extensively in FNA Cytology and Veterinary Medicine. The entire procedure takes 30 seconds as air dried specimens are fixed in Solution 1 then dipped in Solutions 2 and 3. Staining intensity is easily manipulated. Three step procedure for hematological staining, FNA staining, and used as a special stain for helicobacter pylori.
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Unit: Kit Price: $195
The kit components:
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NovaUltraTM Luxol Fast Blue Stain Kit | |
This kit is designed for staining myelin/myelinated axons on
formalin-fixed, paraffin-embedded brain and spinal cord
tissue sections, as well as frozen sections. The myelin,
including phospholipids, will be stained blue to green, and
the neurons will be stained violet.
This stain is
commonly used for identifying the basic neuronal structure
in brain or spinal cord tissue.
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Unit: Kit Price: $195
The kit components:
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NovaUltraTM Masson Trichrome Stain Kit | |
This kit is used for the detection of collagen fibers in tissues such as skin, heart, etc. on formalin-fixed, paraffin-embedded sections, and may be used for frozen sections as well. The collagen fibers will be stained blue and the nuclei will be stained black and the background is stained red. |
Unit: Kit Price: $295
The kit components:
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NovaUltraTM Nissl Stain Kit | |
This kit is used for the detection of Nissl body in the cytoplasm of neurons on formalin-fixed, paraffin embedded tissue sections, as well as frozen sections. The Nissl body will be stained purple-blue. This stain is commonly used for identifying the basic neuronal structure in brain or spinal cord tissue.
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Unit: Kit Price: $95
The kit components:
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NovaUltraTM Oil Red O Stain Kit | |
This kit is for lipid and fat staining on formalin fixed frozen sections. It is not suitable for paraffin embedded tissue sections.
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Unit: Kit Price: $195
The kit components:
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NovaUltraTM Orcein Elastin Stain Kit | |
This kit is used for identifying elastic fibers on formalin-fixed, paraffin-embedded tissue sections, and may be used for frozen sections as well. The elastic fibers will be stained dark brown and the nuclei will be stained light blue. The Acid Orcein Solution has also been show to stain the rough endoplasmic reticulum of hepatitis B infected liver cells (ground glass cells).
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Unit: Kit Price: $195
The kit components:
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NovaUltraTM PAS Stain Kit | |
This kit is used for detection of glycogen in tissues such as liver, cardiac and skeletal muscle on formalin-fixed, paraffin-embedded tissue sections, and may be used for frozen sections as well. The glycogen, mucin, and fungi will be stained purple and the nuclei will be stained blue.
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Unit: Kit Price: $195
The kit components:
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NovaUltraTM Prussian Blue Stain Kit | |
Prussian blue reaction
involves the treatment of sections with acid solutions of ferrocyanides. Any ferric ion (+3) present in the tissue
combines with the ferrocyanide and results in the formation
of a bright blue pigment called Prussian blue. This is one of the most sensitive
histochemical tests and will demonstrate even single
granules of iron in blood cells.
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Unit: Kit Price: $195
The kit components:
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NovaUltraTM Safranin O Stain Kit | |
This kit is used for the detection of cartilage, mucin, and mast cell granules on formalin-fixed, paraffin-embedded tissue sections, and may be used for frozen sections as well. The cartilage and mucin will be stained orange to red, and the nuclei will be stained black. The background is stained green.
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Unit: Kit Price: $295
The kit components:
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NovaUltraTM Picro-Sirius Red Stain Kit | |
It's one of the best understood techniques of collagen histochemistry.
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Unit: Kit Price: $195
The kit components:
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NovaUltraTM Sudan Black B Lipid Stain Kit | |
This kit is for lipid and fat staining on formalin fixed frozen sections. It is not suitable for paraffin embedded tissue sections. The lipid and fat will be stained blue-black and cell nuclei will be stained red.
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Unit: Kit Price: $195
The kit components:
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NovaUltraTM Toluidine Blue Stain Kit | |
Mast
cells are found in the connective tissue and their cytoplasm
contains granules (metachromatic) composed of heparin and
histamine. This kit is designed for staining of formalin
fixed, paraffin embedded tissue sections. Mast cells will be
stained dark blue/red purple (metachromatic staining) and the
background will be stained light blue (orthochromatic staining).
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Unit: Kit Price: $125
The kit components:
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NovaUltraTM von Kossa Stain Kit | |
This technique is for demonstrating
deposits of calcium or calcium salt so it is not specific
for the calcium ion itself. In this method, tissue sections
are treated with a silver nitrate solution and the silver is
deposited by replacing the calcium reduced by the strong
light, and thereby visualized as metallic silver.
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Unit: Kit Price: $195
The kit components:
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How to Order
PO can be Emailed to sales@ihcworld.com or Faxed to 443-638-0394
Credit Card Order need to call us at 443-686-9618
Online Order can be placed on our Online Store at http://store.ihcworld.com/
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References
1. Horiuchi M, et al. Interferon regulatory factor 8/interferon consensus sequence binding protein is a critical transcription factor for the physiological phenotype of microglia. J Neuroinflammation. 2012; 9: 227. Published Online.
2. Blume C, et al. Autoimmunity in CD73/Ecto-5′-Nucleotidase Deficient Mice Induces Renal Injury. PLOS One. 2012; 7(5): e37100. Published Online.
3. Sun H, et al. AAV-mediated Netrin-1 overexpression increases peri-infarct blood vessel density and improves motor function recovery after experimental stroke
. Neurobiol Dis. 2011 October; 44(1): 73–83. Published Online.