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Special Stain Protocols
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Special Stain Protocols
Modified Highman’s Congo Red Staining Protocol for Amyloid
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Description: This modified Highman's Congo Red stain. It is used for the
Bielschowsky’s Silver Staining Protocol for Nerve Fibers, Axons, Neurofibrillary Tangles and Senile Plaques
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Principle: The nerve fibers are sensitized with a silver solution. The
Azure A Staining Protocol for Mast Cells
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Prepared by ROY ELLIS IMVS Division of Pathology The Queen
Burstone’s Staining Protocol for Alkaline Phosphatase on Frozen Tissue Sections
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Solutions and Reagents: Naphthol AS-MX Phosphate/(DMF)N,N Dimethylformamide Solution:       Naphthol AS-MX
Alizarin Red S Staining Protocol for Calcium
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Description: Alizarin Red S, an anthraquinone derivative, may be used to
Alcian Blue and PAS Staining Protocol
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Prepared by ROY ELLIS IMVS Division of Pathology The Queen
Alcian Blue Staining Protocol
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Prepared by ROY ELLIS IMVS Division of Pathology The Queen
Alcian Blue Staining Protocol
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Description: Alcian blue stains acid mucosubstances and acetic mucins. Excessive amounts
Acid Fast Bacilli Staining Protocol
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Prepared by ROY ELLIS IMVS Division of Pathology The Queen
H&E Staining Method and Protocol – Mayer’s
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Solutions and Reagents: Eosin-Phloxine B Solution:       Eosin Stock Solution:       Eosin
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