Scotts Tap Water Substitute
- Magnesium sulfate (MgSO4) ---------- 30.0 g
- Sodium bicarbonate ------------------- 2.0 g
- Tap water ------------------------------ 3L
- Mix well
Scotts Tap Water Substitute is a bluing reagent for histology and cytology staining. It blues hematoxylin gently. It is used as bluing agent for Gill’s hematoxylin H&E staining.
0.1% Sodium Bicarbonate:
- Sodium bicarbonate --------------------------- 1 g
- Distilled water --------------------------------- 1000 ml
Mix to dissolve. The pH will be around 8.0 Store this solution at room temperature.
Note: this solution worked better than ammonia water solution according to testing result.
Bluing for 30 seconds to 1 minute after hematoxylin staining and clearing/differentiation.
0.2% Ammonia Water Solution (Bluing):
- Ammonium hydroxide (concentrated) ------ 2 ml
- Distilled water -------------------------------- 1000 ml
Mix well. The pH will be around 10.0 Store this solution at room temperature.
Note: this solution is not as good as sodium bicarbonate according to testing result.
Bluing for 30 seconds to 1 minute after hematoxylin staining and clearing/differentiation.
Lithium Carbonate Solution (Saturated):
- Lithium carbonate ---------------------------- 1.54 g
- Distilled water -------------------------------- 100 ml
Mix to dissolve and store at room temperature.
Bluing for 30 seconds to 1 minute after hematoxylin staining and clearing/differentiation.