Polink-2 Plus IHC Detection System

 

Most Innovative and Complete

Immunohistochemistry (IHC) Detection Systems

 

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The place to purchase our products online

 

Polink-2 Plus IHC Detection System

New Generation of 2-step Polymer Detection System

for Immunohistochemistry

 

Features

  • Newest Polymer Conjugation Technology
  • Biotin free
  • Ultra Sensitive and High Specificity
  • 10'+10' incubation

Current 2-step polymer-HRP and polymer-AP detection system are widely accepted in the field of immunohistochemistry. However, the current 2-step polymer detection system demonstrate 3 weaknesses: 1. It can only enhance the signal for mouse primary antibodies and does not enhance the signal for rabbit primary antibodies. 2. The current 2-step polymer detection system offers customers only for broad spectrum (for mouse and rabbit) due to the limitation of the bridging antibody. 3. The current 2-step polymer system does not provide satisfactory sensitivity when detecting rabbit antibodies, goat antibodies and rat antibodies. Through new conjugation technology, Polink-2 Plus Series overcomes the weaknesses of current 2-step polymer detection systems. It demonstrates ultra sensitivity and high specificity to mouse, rabbit, goat and rat antibodies. This system reduces incubation time by 10 minutes for each incubation step, saving a total of 20-30 minutes for the entire process.

 


 

Polink-2 Plus Polymer HRP or AP Detection Kits for

Broad Spectrum (Mouse & Rabbit Primary Antibody)

 

Polink-2 Plus HRP DAB Broad kit stained mouse anti-ER on human breast cancer tissue.

 

Polink-2 Plus Detection Kits for broad spectrum (mouse and rabbit) demonstrate excellent sensitivity to mouse and rabbit antibody with choice of Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP) conjugate. This innovative technology surpasses other 2-step polymer technologies when detecting rabbit antibodies. It’s super sensitivity allows customers to detect difficult-to-work antibodies.

 

Polink-2 Plus HRP Broad kit stained rabbit anti glucagon 1:400 on human pancreas.

A leading competitor’s 2-step polymer kitstained rabbit anti glucagon 1:400 on humanpancreas.

 

Catalog No.

Product Name

Serum Adsorbed

Chromogen

D41-110

Polink-2 Plus HRP Broad for DAB Bulk kit, 110 ml

Human

Not included

D41-15

Polink-2 Plus HRP Broad with DAB Kit, 15 ml

Human

DAB

D41-6

Polink-2 Plus HRP Broad with DAB Kit, 6 ml

Human

DAB

D42-110

Polink-2 Plus HRP Broad for AEC Bulk kit, 110 ml

Human

Not included

D42-15

Polink-2 Plus HRP Broad with AEC kit, 15 ml

Human

AEC

D42-6

Polink-2 Plus HRP Broad with AEC kit, 6 ml

Human

AEC

D68-110

Polink-2 Plus AP Broad Bulk kit, 110 ml

Human

Not included

D68-15

Polink-2 Plus AP Broad kit, 15 ml

Human

Not included

D68-6

Polink-2 Plus AP Broad kit, 6 ml

Human

Not included

 

 

Polink-2 Plus Polymer HRP or AP Detection Kits for

Mouse Primary antibody

 

Mouse anti B Cell 1:400 stained Tonsil by Polink-2 Plus Mouse DAB Kit (Cat. No. D37-15)

 

Polink-2 Plus Detection Mouse Kits demonstrate excellent sensitivity to mouse antibody with choice of Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP) conjugate. This innovative technology surpasses other polymer technologies by 2-4 fold when detecting difficult-to-work antibodies.

 

Mouse anti ER 1:400 by Polink-2 Plus HRP Mouse DAB Kit (Cat. No. D37-15)

Mouse anti ER 1:100 by a leading company’s polymer detection system


Catalog No.

Product Name

Serum Adsorbed

Chromogen

D37-110

 

Polink-2 Plus HRP MOUSE for DAB Bulk Kit, 110 ml

Human, Rabbit

Not included

D37-15

 

Polink-2 Plus HRP MOUSE with DAB kit, 15 ml

Human, Rabbit

DAB

D37-6

 

Polink-2 Plus HRP MOUSE with DAB kit, 6 ml

Human, Rabbit

DAB

D38-110

 

Polink-2 Plus HRP MOUSE for AEC Bulk kit, 110 ml

Human, Rabbit

Not included

D38-15

 

Polink-2 Plus HRP MOUSE with AEC Kit, 15 ml

Human, Rabbit

AEC

D38-6

 

Polink-2 Plus HRP MOUSE with AEC Kit, 6 ml

Human, Rabbit

AEC

D69-110

 

Polink-2 Plus AP MOUSE Bulk kit, 110 ml

Human, Rabbit

Not included

D69-15

 

Polink-2 Plus AP MOUSE kit, 15 ml

Human, Rabbit

Not included

D69-6

 

Polink-2 Plus AP MOUSE kit, 6 ml

Human, Rabbit

Not included


 

Polink-2 Plus Polymer HRP or AP Detection Kits for

Rabbit Primary Antibody

 

Polink-2 Plus HRP or AP Rabbit Detection Kit is an ultra sensitive polymer detection system. It uses rabbit antibody specific enhancer to help amplify the polymer-enzyme conjugate reaction to achieve super sensitivity and specificity in immunohistochemistry staining. It produces 2-4 fold sensitivity compared to other 2-step polymer detection system on the market. User may need to further dilute primary antibody due to super sensitivity of Polink-2 Plus detection system. It is a biotin-free system, therefore, overcomes the non-specific staining caused by streptavidin /biotin system due to endogenous biotin. Most commonly used specimens for this system are: frozen tissue, paraffin-embedded tissue, freshly prepared lymphocytes and fixed culture cells. It is compatible either with manual or automated systems. Staining conditions need to be optimized by user.

 

Rabbit anti S-100 1:400 stained on human skin by Polink-2 Plus HRP DAB Rabbit Kit (Cat. D39-15)

Rabbit anti S-100 1:400 stained on human skin by 2-step polymer HRP kit from a leading competitor

 

Catalog No.

Product Name

Serum Adsorbed

Chromogen

D39-110

Polink-2 Plus HRP RABBIT for DAB Bulk Kit, 110 ml

Human, Mouse

Not included

D39-15

Polink-2 Plus HRP RABBIT with DAB kit, 15 ml

Human, Mouse

DAB

D39-6

 

Polink-2 Plus HRP RABBIT with DAB kit, 6 ml

Human, Mouse

DAB

D40-110

 

Polink-2 Plus HRP RABBIT for AEC Bulk kit, 110 ml

Human, Mouse

Not included

D40-15

 

Polink-2 Plus HRP RABBIT with AEC kit, 15 ml

Human, Mouse

AEC

D40-6

 

Polink-2 Plus HRP RABBIT with AEC kit, 6 ml

Human, Mouse

AEC

D70-110

 

Polink-2 Plus AP Rabbit Bulk kit, 110 ml

Human, Mouse

Not included

D70-15

 

Polink-2 Plus AP Rabbit kit, 15 ml

Human, Mouse

Not included

D70-6

 

Polink-2 Plus AP Rabbit kit, 6 ml

Human, Mouse

Not included

 

 

Polink-2 Plus Polymer HRP or AP Detection Kits for

Goat Primary Antibody

 

Polink-2 Plus HRP or AP Goat Detection Kit is an ultra-sensitive polymer detection system. It uses Goat antibody specific enhancer to help amplify the polymer-enzyme conjugate reaction to achieve super sensitivity and specificity in immunohistochemistry staining. It produces consistent immunostaining outcomes on archival tissues and on difficult-to-work antibodies. User may need to further dilute primary antibody due to the super sensitivity of Polink-2 Plus detection system. It is a biotin-free system, therefore, overcomes the non-specific staining caused by streptavidin/biotin systems due to endogenous biotin. Most commonly used specimens for this system are: frozen tissue, paraffin-embedded tissue, freshly prepared lymphocytes and fixed culture cells. It is compatible with either manual or automated systems. Staining conditions need to be optimized by user.

 

Goat anti P53 1:200 stained on human

breast cancer by Polink-2 Plus HRP

Goat AEC Kit (Cat. No.D45-15)

Goat anti P53 1:200 stained on human

breast cancer by 1-step polymer HRP

Goat AEC kit from a leading competitor

 

Catalog No.

Product Name

Serum Adsorbed

Chromogen

D43-110

 

Polink-2 Plus HRP GOAT for DAB Bulk kit, 110 ml

Human, Mouse, Rabbit

Not included

D43-15

 

Polink-2 Plus HRP GOAT with DAB kit, 15 ml

Human, Mouse, Rabbit

DAB

D43-6

Polink-2 Plus HRP GOAT with DAB kit, 6 ml

Human, Mouse, Rabbit

DAB

D45-110

 

Polink-2 Plus HRP GOAT for AEC Bulk kit, 110 ml

Human, Mouse, Rabbit

Not included

D45-15

 

Polink-2 Plus HRP GOAT with AEC kit, 15 ml

Human, Mouse, Rabbit

AEC

D45-6

Polink-2 Plus HRP GOAT with AEC kit, 6 ml

Human, Mouse, Rabbit

AEC

D66-110

 

Polink-2 Plus AP GOAT Bulk kit, 110 ml

Human, Mouse, Rabbit

Not included

D66-15

 

Polink-2 Plus AP GOAT kit, 15 ml

Human, Mouse, Rabbit

Not included

D66-6

Polink-2 Plus AP GOAT kit, 6 ml

Human, Mouse, Rabbit

Not included

 


Polink-2 Plus Polymer HRP or AP Detection Kits for

Rat Primary antibody

 

Detecting RAT primary antibody on MOUSE tissue is a very difficult task in research field due to background staining issues. Polink-2 Plus HRP or AP Rat-NM Detection Kit is specially designed to solve the problem. The secondary antibody is adsorbed to mouse and human serum proteins. This technology provides excellent specificity to detect rat primary antibody (user supplied) on mouse tissue. Besides mouse tissue Polink-2 Plus Rat-NM Detection kit also can be used on human tissue as well.

Polink-2 Plus Rat-NM Detection Kit uses rat antibody enhancer to help amplify the polymer-enzyme conjugate reaction to achieve super sensitivity and specificity in immunohistochemistry staining. It produces consistent immunostaining outcomes on archival tissues and on difficult-to-work antibodies. User may need to further dilute primary antibody due to super sensitivity of Polink-2 Plus detection system. It is a biotin-free system, therefore it overcomes the non-specific staining caused by streptavidin/biotin system due to endogenous biotin. Most commonly used specimens for this system are: frozen tissue, paraffin-embedded tissue, freshly prepared lymphocytes and fixed culture cells. It is compatible with either manual or automated systems. Staining conditions need to be optimized by user.


Rat anti Podoplanin 1:800 stained on human skin by Polink-2 Plus HRP Rat-NM DAB kit (Cat. No. D46-15)

Rat anti Podoplanin 1:800 stained on human skin by 1-step polymer HRP kit

 

Catalog No.

Product Name

Serum Adsorbed

Chromogen

D46-110

Polink-2 Plus HRP RAT-NM for DAB bulk Kit, 110 ml

No cross react to mouse

Not included

D46-15

 

Polink-2 Plus HRP RAT-NM with DAB kit, 15 ml

No cross react to mouse

DAB

D46-6

Polink-2 Plus HRP RAT-NM with DAB kit. 6 ml

No cross react to mouse

DAB

D48-110

 

Polink-2 Plus HRP RAT-NM for AEC bulk Kit, 110 ml

No cross react to mouse

Not included

D48-15

 

Polink-2 Plus HRP RAT-NM with AEC Kit, 15 ml

No cross react to mouse

AEC

D48-6

 

Polink-2 Plus HRP RAT-NM with AEC Kit, 6 ml

No cross react to mouse

AEC

D67-110

 

Polink-2 Plus AP RAT-NM Bulk Kit, 110 ml

No cross react to mouse

Not included

D67-15

 

Polink-2 Plus AP RAT-NM kit, 15 ml

No cross react to mouse

Not included

D67-6

Polink-2 Plus AP RAT-NM kit, 6 ml

No cross react to mouse

Not included

 

 

Polink-2 Plus Polymer HRP or AP Detection Kits for

Mouse Primary antibody on Rat tissue

 

Detecting MOUSE primary antibody on RAT tissue is a very difficult task in research field due to background staining issues. Polink-2 Plus HRP or AP Mouse-NR (no cross react to rat) Detection Kit is specially designed to solve the problem. The secondary antibody is adsorbed to rat, rabbit and human serum proteins. This technology provides excellent specificity to detect mouse primary antibody (user supplied) on rat tissue. Besides rat tissue Polink-2 Plus Mouse-NR Detection kit also can be used on human tissue and rabbit tissue as well.

 

Polink-2 Plus Mouse-NR Detection Kit is a ultra sensitive polymer detection system. It uses mouse antibody enhancer to help amplify the polymer-enzyme conjugate reaction to achieve super sensitivity and specificity in immunohistochemistry staining. It produces consistent immunostaining outcomes on archival tissues and on difficult-to-work antibodies. User may need to further dilute primary antibody due to super sensitivity of Polink-2 Plus detection system. It is a biotin-free system, therefore it overcomes the non-specific staining caused by streptavidin/biotin system due to endogenous biotin. Most commonly used specimens for this system are: frozen tissue, paraffin-embedded tissue, freshly prepared lymphocytes and fixed culture cells. It is compatible with either manual or automated systems. Staining conditions need to be optimized by user.

 

Mouse anti S-100 on RAT skin stained by Polink-2 Plus AP Mouse-NR Kit (Cat.No. D65-15)

Mouse anti S-100 on RAT brain stained by Polink-2 Plus AP Mouse-NR Kit (Cat.No. D65-15)

 

Catalog No.

Product Name

Serum Adsorbed

Chromogen

D58-110

 

Polink-2 Plus HRP MOUSE-NR for DAB Bulk Kit, 110 ml

No cross react to rat

Not included

D58-15

 

Polink-2 Plus HRP MOUSE-NR with DAB Kit, 15 ml

No cross react to rat

DAB

D58-6

Polink-2 Plus HRP MOUSE-NR with DAB Kit, 6 ml

No cross react to rat

DAB

D59-110

 

Polink-2 Plus HRP MOUSE-NR for AEC Bulk Kit, 110 ml

No cross react to rat

Not included

D59-15

 

Polink-2 Plus HRP MOUSE-NR with AEC Kit, 15 ml

No cross react to rat

AEC

D59-6

Polink-2 Plus HRP MOUSE-NR with AEC Kit, 6 ml

No cross react to rat

AEC

D65-110

 

Polink-2 Plus AP MOUSE-NR Bulk Kit, 110 ml

No cross react to rat

Not included

D65-15

 

Polink-2 Plus AP MOUSE-NR kit, 15 ml

No cross react to rat

Not included

D65-6

Polink-2 Plus AP MOUSE-NR kit, 6 ml

No cross react to rat

Not included



 

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