Aqueous Mounting Medium (glycerol gelatin)
Kisser’s | Kaiser's | Glycerol jelly | Modified | |
Gelatine | 10 g | 40 g | 65 g | 5 g |
Distilled water | 35 ml | 210 ml | 300 ml | 50 ml |
Glycerol (glycerin) | 30 ml | 250 ml | 100 ml | 50 ml |
Phenol (carbolic acid) | 5 ml | 5 ml |
Mix gelatin and distilled water. Heat in 50 C oven until gelatin is dissolved (it takes about 30 minutes). Add glycerin and adjust pH to 7.0 using 1N NaOH (to avoid fading of hematoxylin). Store at room temperature.
Warm to 37-40 C before mounting. Note: Modified protocol works well.