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Aqueous Mounting Medium Protocols
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Aqueous Mounting Medium Protocols

Aqueous Mounting Medium (glycerol gelatin)

 Kisser’sKaiser'sGlycerol jellyModified
Gelatine10 g40 g65 g5 g
Distilled water35 ml210 ml300 ml50 ml
Glycerol (glycerin)30 ml250 ml100 ml50 ml
Phenol (carbolic acid) 5 ml5 ml 

Mix gelatin and distilled water. Heat in 50 C oven until gelatin is dissolved (it takes about 30 minutes). Add glycerin and adjust pH to 7.0 using 1N NaOH (to avoid fading of hematoxylin). Store at room temperature.

Warm to 37-40 C before mounting. Note: Modified protocol works well.

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