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Protocols
Immunofluorescence Staining Protocol
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 1. Preparation of Slides  A. Cell Lines B. Frozen Sections C.
Immunohistochemistry Enzyme Alkaline Phosphatase (AP) Staining Protocol
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1. Preparation of Slides  A. Cell Lines  B. FrozenSections Snap frozen
Immunohistochemistry Enzyme HRP Staining Protocol
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1. Preparation of Slides  A. Cell Lines  B. FrozenSections Snap frozen
Blocking of Unwanted Non-specific Staining for Immunohistochemistry
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Dr. Giorgio Gattoretti Associate Professor of Clinical PathologyInstitute for Cancer
Apoptosis Detection Using Terminal Transferase and Biotin-16-dUTP (TUNEL Fluorescent Method)
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Description: This protocol is used for detection and quantification of apoptosis
Apoptosis Detection Using Terminal Transferase and Biotin-16-dUTP (TUNEL Enzyme Method)
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Description: This protocol is used for detection and quantification of apoptosis
Antigen Retrieval Protocols – Universal Antigen Retrieval Method
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IHC-Tek Reagent - IHC-Detection System - IHC Staining System -
Antigen Retrieval Method for Free Floating Sections
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Description: We describe here a simple, reliable and sensitive antigen retrieval
Antigen Retrieval Method for Frozen Sections
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Description: Although AR by heating is widely effective, the majority ofthe
Antigen Retrieval Method for Cryostat Frozen Sections
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Description: This is a simple method for antigen retrieval on aldehyde-fixed
Protease Antigen Retrieval Protocol
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Description: Formalin or other aldehyde fixation forms protein cross-links that mask
Pronase Antigen Retrieval Protocol
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Description: Formalin or other aldehyde fixation forms protein cross-links that mask