Image Analysis

 

 

 

Quantitative Scoring Methods

 

1

Score 0 1+ 2+ 3+ 4+
Positive Cells <10% 10-25% 25-50% 50-75% >75%

2

Score 0 1 2 3 4
Positive Cells negative few nuclei 10% 10-50% >50%

3

Score low level high level  
Positive Cells <20% >20%  

4

Score 1 2 3
Intensity of Staining weak staining moderate staining strong staining

5 Quick score (Q):

Results are scored by multiplying the percentage of positive cells (P) by the intensity (I). 

Formula: Q = P x I; Maximum = 300

 

References

 

McDonald JW, Pilgram TK. Nuclear expression of p53, p21 and cyclin D1 is increased in bronchioloalveolar carcinoma. Histopathology. 1999 May;34(5):439-46

 

Shiao YH, Palli D, Caporaso NE, Alvord WG, Amorosi A, Nesi G, Saieva C, Masala G, Fraumeni JF Jr, Rice JM. Genetic and immunohistochemical analyses of p53 independently predict regional metastasis of gastric cancers. Cancer Epidemiol Biomarkers Prev. 2000 Jun;9(6):631-633

 

Charafe-Jauffret E, Tarpin C, Bardou VJ, Bertucci F, Ginestier C, Braud AC, Puig B, Geneix J, Hassoun J, Birnbaum D, Jacquemier J, Viens P. Immunophenotypic analysis of inflammatory breast cancers: identification of an 'inflammatory signature'. J Pathol. 2004 Mar;202(3):265-73