 Methods & Techniques For Histologists and Immunohistochemists |
Image Analysis
Quantitative Scoring Methods
1
| Score | 0 | 1+ | 2+ | 3+ | 4+ |
| Positive Cells | <10% | 10-25% | 25-50% | 50-75% | >75% |
2
| Score | 0 | 1 | 2 | 3 | 4 |
| Positive Cells | negative | few nuclei | 10% | 10-50% | >50% |
3
| Score | low level | high level | |||
| Positive Cells | <20% | >20% |
4
| Score | 1 | 2 | 3 | ||
| Intensity of Staining | weak staining | moderate staining | strong staining |
5 Quick score (Q):
Results are scored by multiplying the percentage of positive cells (P) by the intensity (I).
Formula: Q = P x I; Maximum = 300
IHCscore - Quantitative Tissue Scoring
IHCscore is image analysis software designed for quantitative tissue scoring utilizing WebSlide virtual slides, operating either locally or across a network. When slides containing immunohistochemically (IHC) stained tissue sections are scanned into virtual slides and placed on a network, multiple people can collaborate, visually score or use the quantitative measurements and the built in classifier to automatically score each specimen.
References