In Situ Hybridization - ISH

Fluorescent In Situ Hybridization - FISH

 

 

 

Overview

• What is FISH and how does it work?

• Definition of FISH

• In situ Hybridization Wiki Entry

• FISH (fluorescence in situ hybridization) Wiki Entry

• Introduction to in situe Hybridization (Sean M. Montgomery)

• In Situ Hybridization Introduction (GeneDetect)

Manuals

• Multiplex Fluorescent mRNA In Situ Hybridization (Dave Kosman, UC San Diego)

• Tavi’s Multicolor FISH Page (Yale University)

• Nonradioactive In Situ Hybridization Application Manual (Roche)

Protocols

• Anderson Lab In Situ Hybridization Protocols

• Nonradioactive In Situ Hybridization Methods

• In Situ Hybridization Protocols (Protocol Online)

• SingerLab In Situ Hybridization Protocols

• In Situ Hybridization on Frozen Sections (Josiah N. Wilcox)

• In Situ Hybridization Using Digoxigenin Labeled Probes

• In Situ Hybridization to Paraffin and Frozen Tissue Sections (Kornberg)

• Chromosome In situ Hybridization Using Biotin Labeled Probes

• Non-radioactive in situ hybridization protocol for plants (Jeff Long)

• Protocol for mRNA in situ hybridization with nick-translated probles

• In situ hybridization method (Klymkowsky Lab)

• In situ hybridization protocols (Kansas State University)

• Enhanced two color fluorescent in situ hybridization for low-abundance genes

• Immunohistochemistry and In Situ Hybridization Protocols (Nature Protocols)

• In Situ Hybridization Protocols (Immunoportal)

• FISH Protocols for Drosophila

Whole Mount Protocols

• High-resolution in situ hybridization to whole-mount zebrafish embryos (Nature Protocols)

• Whole Mount In Situ Hybridization Protocol

• Wholemount in situ hybridization protocol (Murray Hargrave, Koopman Lab)

• RNA Whole Mount In situ Hybridization (Cepko/Tabin Lab)

• In situ hybridization on whole mount embryos of C.elegans (NIG, Japan)

• Whole mount in situ hybridization for the detection of mRNA in Drosophila embryos (Roche)

• Zebra fish whole mount in situ hybridization protocols (Roe Lab)

Products and Services

• In Situ Hybridization with Nanogold-Streptavidin

• In situ hybridization reagents (invitrogen)

Videos

• In Situ Hybridization Introduction

• Fluorescent In Situ Hybridization (FISH) Method

Literatures and Articles

• An Optimized Method for In Situ Hybridization with Signal Amplification That Allows the Detection of Rare mRNAs

• A sensitive non-radioactive in situ hybridization method for the detection of chicken IgG γ-chain mRNA: a technique suitable for detecting of variety of mRNAs in tissue sections

• Dual Fluorescent In Situ Hybridization and Immunohistochemical Detection with Tyramide Signal Amplification

• In situ Hybridization Using Antibody-Based Methods

Resources

• GEISHA – Gallus Expression in Situ Hybridization Analysis