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Bombesin Antibody Staining Protocol for Immunohistochemistry
Description: Gastrin-releasing peptide is a regulatory peptide common for mammalian species: the amphibian counterpart is bombesin. Bombesin is expressed in neural cells and in many neuroendocrine cells.
Primary Antibody
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Name: Bombesin Antibody |
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Clone: Polyclonal, Rabbit Anti-Human |
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Supplier: Novocastra Labs |
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Catalog Number: NCL-BOMp |
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Dilution: 1:200 - 1:400 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
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Incubation Time/Temp: 60min/room temperature |
Antigen Retrieval
| Device: Antigen retrieval is not needed |
| Buffer/pH value: N/A |
| Heat/Cool Temperature: N/A |
| Heat/Cool Time: N/A |
Detection Methods
| Standard Method: ABC Method or LSAB Method |
| Enhanced Method: Polymeric Methods |
Chromogen Substrate
| Reagent: DAB |
| Incubation Time/Temperature: 1-3 minutes/room temperature |
Counterstain
| Reagent: Mayer's Hematoxylin |
| Staining Time: 30 seconds |
Results:
| Staining Pattern: Cytoplasmic |
| Images: Search image |
Additional Information:
| Tissue Type: Foetal lung |
| Fixation: Formalin-fixed paraffin sections |
| Positive Control: Foetal lung |
| Negative Control: Omit primary antibody, isotype control, absorption control |
| Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
References: