beta Amyloid, 1-16 (6E10) Antibody Staining Protocol for Immunohistochemistry


Description: This antibody is reactive to amino acid residues 1-16 of beta amyloid. The epitope lies within amino acids 3-8 of beta amyloid (EFRHDS). Beta amyloid forms are deposited in the CNS of patients with Alzheimer’s disease and Down’s syndrome. Signet’s beta amyloid antibody 6E10 reacts to the abnormally processed isoforms, as well as precursor forms.


Primary Antibody

Name: Mouse Anti-beta Amyloid Antibody

Clone: 6E10

Supplier: Covance

Catalog Number: SIG-39300

Dilution: 1:100 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.

Incubation Time/Temp: 60 min/room temperature

Antigen Retrieval
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102)
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100)
Heat/Cool Temperature: 95-100 ºC/room temperature
Heat/Cool Time: 20 minutes/20 minutes
70% formic acid pretreatment for 10 minutes also works well for this antibody.

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB Chromogen Substrate
Incubation Time/Temperature: 1-3 minutes/room temperature

Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Staining Pattern: Amyloid deposites
Images: Search image

Additional Information:
Species Reactivity: Human, mouse
Fixation: Formalin fixed paraffin sections
Positive Control: Alzheimer's brain
Negative Control: Omit primary antibody, isotype control, absorption control
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary