IMVS Division of Pathology
The Queen Elizabeth Hospital
Woodville Road, Woodville, South Australia 5011
PROBLEM NUMBER 6
Shattering, cracked or folded sections from large blocks processed through paraffin wax.
This is a section of kidney stained Haematoxylin and Eosin showing significant cracking and staining very red.
This is a result of overdehydration. Too much moisture has been removed from the block during processing. Strangely enough cutting a good section does require that some moisture remain in the tissue.
The solution is to place a soaked cotton gauze over the block face for 5 to 10 seconds before cutting. This allows enough moisture to penetrate 15 to 20 µm allowing a quality section to be cut.
Click the button to return to the Home Page
© Roy C. Ellis 2002