Problems and Solutions in Histological Technique

 

Problems in Histopathological Technique

 

Prepared by

ROY ELLIS

IMVS Division of Pathology

The Queen Elizabeth Hospital

Woodville Road, Woodville, South Australia 5011

Email: roy.ellis@imvs.sa.gov.au

 

 

 

PROBLEM NUMBER 29

Poor staining or lack of staining with alcian blue dissolved in acetic acid and commonly used for staining mucin.

 

WHY?

 

Alcian blue is said to denature in acid solution over a period of time, the stronger the acid component the sooner denaturation occurs. For example it is said that in a 3% acetic acid solution alcian blue denatures in approximately 1 month.

 

So the answer is to make up the solution more frequently.

 

 

 

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© Roy C. Ellis 2002