Problems in Histopathological Technique

 

Prepared by

ROY ELLIS

IMVS Division of Pathology

The Queen Elizabeth Hospital

Woodville Road, Woodville, South Australia 5011

Email: roy.ellis@imvs.sa.gov.au

 

 

 

PROBLEM NUMBER 19

Sections disintegrate on the water bath

 

WHY?

  • This can happen when the water temperature is too high. It has a tendency to soften and melt the wax and once the wax breaks up so does the section.

  • If the wax is contaminated with clearing agent or if the block has not been properly dehydrated then the section will also disintegrate. We discussed this one also when we talked about the problems associated with sections crumbling.

           

 

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© Roy C. Ellis 2002