Problems and Solutions in Histological Technique

 

Problems in Histopathological Technique

 

Prepared by

ROY ELLIS

IMVS Division of Pathology

The Queen Elizabeth Hospital

Woodville Road, Woodville, South Australia 5011

Email: roy.ellis@imvs.sa.gov.au

 

 

 

DID YOU KNOW 3?

Did you know that pseudocalcification artefacts are seen when calcium acetate or carbonate is used to neutralise 10% formalin or when any calcium salts are used in compound fixatives?

 

Formol calcium would be the most common and is a fixative which Baker originally recommended as one which preserved the structure of phospholipids better than formalin alone or formalin in combination with sodium chloride.


And I think you will agree this section of kidney fixed in formol calcium and stained H&E shows pretty good, well preserved cellular detail and excellent staining as well as showing calcium deposits.

 

 

This is a section from the same kidney as the previous section, so it was fixed in formol calcium and stained by Von Kossa showing the distribution of the black calcium deposits.

 

 

 

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© Roy C. Ellis 2002