Problems in Histopathological Technique
IMVS Division of
Woodville, South Australia 5011
DID YOU KNOW 16?
Did you know that when solutions are heated in a
microwave for fixation or staining there can be marked
differences in temperature between the top and bottom layers of
That difference can be as much as 15°C.
So take care when using microwave techniques that the
recommended temperature is the one that you are using. A microwave
that has a means for inserting a temperature probe which can be
placed in the solution at the level of the section is the best
insurance. Otherwise a frequent check of the temperature after
stirring the solution should do the trick.
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© Roy C. Ellis 2002