Problems and Solutions in Histological Technique

 

Problems in Histopathological Technique

 

Prepared by

ROY ELLIS

IMVS Division of Pathology

The Queen Elizabeth Hospital

Woodville Road, Woodville, South Australia 5011

Email: roy.ellis@imvs.sa.gov.au

 

 

 

DID YOU KNOW 13?
Did you know that nucleoli stain blue after Zenker fixation?
Nucleoli are composed mainly of RNA and usually stain red after eosin staining in a Haematoxylin and Eosin. So it is unusual that after Zenker fixation they stain blue. Try it out on a piece of tissue from a tumour, where cells are very active and you should find many blue nucleoli but they can sometimes be difficult to detect in a nucleus that also stains the same colour.



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© Roy C. Ellis 2002