Problems in Histopathological Technique
IMVS Division of
Woodville, South Australia 5011
DID YOU KNOW 12?
Did you know that bone specimens can be processed
undecalcified and then surface decalcified in 10% aqueous
hydrochloric acid for 15 to 30 minutes before cutting. Calcified
specimens can thus be available more quickly for diagnosis and
staining is almost always better than if the bone is first
This is a low power Haematoxylin and Eosin stained section of
bone that was processed undecalcified and surface decalcified
before cutting a section.
Processed this way there is very little shrinkage of soft tissue
components away from the compact bone as is often the case when
decalcification is done before processing. The overall staining is
quite good and sections really are easy to obtain. Immunoperoxidase
staining is far better also if bone is not decalcified for lengthy
periods. But blocks need to be washed before cutting because
decalcifying fluids quickly destroy knife edges.
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© Roy C. Ellis 2002