Problems in Histopathological Technique
IMVS Division of
Woodville, South Australia 5011
DID YOU KNOW 1?
Did you know that Hyaluronic acid cannot be demonstrated
after tissues are fixed in Zenker’s or Bouin’s fluids?
DO YOU CARE?
Probably not if you work in a routine laboratory – but it can be
important if you work in a research laboratory where this type
of fixative is more commonly used to fix and enhance particular
structures. Prolonged washing is also required after both - to
remove the dichromate from Zenker and the picric acid from
Bouin's which makes both unsuitable for a busy routine
Why is the next question and the answer is because most
mucosubstances are hydrophilic and can be denatured by aqueous
fixatives like Zenker and Bouin's.
The most effective method of fixation for all mucosubstances is
any of the alcoholic fixatives that have been pre-cooled to
below -50°C. The snap frozen tissue can then be allowed to thaw
to room temperature in the fixative. Hydrophilic structures,
like mucosubstances, are thus immobilised within the tissue for
subsequent processing like dehydration, clearing and embedding.
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© Roy C. Ellis 2002