Polink-2 IHC Detection System

 

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Immunohistochemistry (IHC) Detection Systems

 

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Polink-2 HRP Detection System

Two-step polymer-HRP detection system

for Mouse & Rabbit antibodies

 

Comparison of immunostaining intensity using Polink-2 HRP detection kit with other source polymer detection kit. A and B are colon cancer stained by Ms anti-CEA. C and D are breast cancer stained by Ms anti-ER. B and D are detected using Polink-2 HRP (cat. No. D22-15) kit. A and C are detected using other supplier’s polymer kit. The intensity of immunostaining using Polink-2 HRP kit is significantly stronger than other source polymer kit.

 

Features

  • 2nd generation of polymer HRP (non-biotin) system, avoids endogenous biotin background completely

  • More sensitive than Polink-1 HRP system with clean background

  • Two-step polymer detection system

  • Compatible with either manual or automated systems

  • Better results on difficult-to-work antibodies and nuclear staining

  • Save cost due to further dilution of primary antibody

Polink-2 HRP Detection Kit is the second generation of polymer-based detection system that uses proprietary polymer helper and polymeric HRP-linked secondary antibody conjugates to achieve super sensitivity and consistent immunostaining.

 

This technology provides excellent sensitivity and high specificity. It is about 3-4 times more sensitive than the 1-step polymer detection system and biotin-streptavidin system. It helps to achieve better results on difficult-to-work antibody and nuclear staining. It is a biotin-free system, so therefore overcomes the non-specific staining caused by streptavidin/biotin system due to endogenous biotin. Since it has higher sensitivity than other conventional methods and the 1-step polymer detection system (Polink-1 HRP system), users may need to further dilute the concentration of primary antibodies if switching from conventional three-step or Polink-1 HRP detection methods.

 

Refer to the following table to choose Polink-2 HRP for DAB or Polink-2 HRP for AEC. For single species capacity or ultra sensitivity, please choose the third generation of polymer detection system, Polink-2 Plus HRP detection system.

 

 

Catalog No.

Product Name

Primary Antibody

Chromogen

D22-110

Polink-2 HRP Broad for DAB Bulk kit, 110 ml

Mouse & Rabbit

Not included

D22-60

Polink-2 HRP Broad for DAB Bulk kit, 60 ml

Mouse & Rabbit

Not included

D22-15

Polink-2 HRP Board with DAB, 15 ml

Mouse & Rabbit

DAB

D22-6

Polink-2 HRP Board with DAB, 6 ml

Mouse & Rabbit

DAB

D23-110

Polink-2 HRP Board for AEC Bulk kit, 110 ml

Mouse & Rabbit

Not included

D23-60

Polink-2 HRP Board for AEC Bulk kit, 60 ml

Mouse & Rabbit

Not included

D23-15

Polink-2 HRP Board with AEC, 15 ml

Mouse & Rabbit

AEC

D23-6

Polink-2 HRP Board with AEC, 6 ml

Mouse & Rabbit

AEC

 

Polink-2 AP Detection System

Two-step polymer-AP detection system

for Mouse & Rabbit antibodies

 

Ms. Anti-ER stained breast cancer using Polink-2 AP kit (Cat No D24-110) with Fast red.

Rb. Anti-PLAP stained placenta using Polink-2 AP kit (Cat No. D24-110) with Fast Red.

 

Features

  • 2nd generation of polymer AP (non-biotin) system, avoids endogenous biotin background completely

  • More sensitive than Polink-1 AP system with clean background

  • Two-step polymer detection system

  • Compatible with either manual or automated systems

  • Better results on difficult-to-work antibodies and nuclear staining

  • Save cost due to further dilution of primary antibody

Polink-2 AP detection kit is the second generation polymer-based detection system that uses proprietary polymer helper and polymeric alkaline phosphatase (AP)-linked secondary antibody conjugates to achieve super sensitivity and consistent immunostaining.

 

This technology provides excellent sensitivity and high specificity. It is about 3-4 times more sensitive than the 1-step polymer-AP detection system and biotin-streptavidin system. It helps to achieve better results on difficult-to-work antibody and nuclear staining. It is a biotin-free system, so therefore overcomes the non-specific staining caused by streptavidin/biotin system due to endogenous biotin. Since it has higher sensitivity than other conventional methods and 1-step polymer detection system (Polink-1 AP system), users may need to further dilute the concentration of primary antibodies if switching from the conventional three-step or Polink-1 AP detection methods.

 

Only broad spectrum detection kits are available in Polink-2 AP detection system. For working with single species or ultra sensitivity, please choose the third generation of polymer detection system, Polink-2 Plus AP detection system.

 

 

Catalog No.

Product Name

Primary Antibody

Chromogen

D24-110

Polink-2 AP Board Bulk Kit, 110 ml

Mouse & Rabbit

Not included

D24-15

Polink-2 AP 15ml Kit

Mouse & Rabbit

Not included

D24-6

Polink-2 AP 6ml kit

Mouse & Rabbit

Not included



 

How to Order


 

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