Description

Annexin V also know as endonnexin II (ENX2）,or placental protein 4 (PP4). Endonexin II is a member of the family of Ca(2+)-dependent phospholipid binding proteins, known as annexins.  It binds to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. It has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2 (basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The PP4 cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. PP4 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Catalog Number

IW-PA1008

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide corresponding to a sequence near the C-terminal of human Annexin V, identical to the related rat and mouse sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Rat cardiac muscle

Cellular Localization

Membrane, cytoplasmic.

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Proteinase K digestion is required on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Suitable

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Kaplan, R.; Jaye, M.; Burgess, W. H.; Schlaepfer, D. D.; Haigler, H. T. : Cloning and expression of cDNA for human endonexin II, a Ca(2+) and phospholipid binding protein. J. Biol. Chem. 263

2. Rodriguez-Garcia, M. I.; Kozak, C. A.; Morgan, R. O.; Fernandez, M. P. : Mouse annexin V chromosomal localization, cDNA sequence conservation, and molecular evolution. Genomics 31: 151-157, 1996.

3. Grundmann, U.; Abel, K.-J.; Bohn, H.; Lobermann, H.; Lottspeich, F.; Kupper, H. : Characterization of cDNA encoding human placental anticoagulant protein (PP4): homology with the lipocortin family. Proc. Nat. Acad. Sci. 85: 3708-3712, 1988.

Images

Immunohistochemical analysis of paraffin-embedded sections

Rat cardiac muscle tissue using Annexin V antibody