Antibody Amplifier

An Innovative Immunohistochemistry Staining System

 

The Antibody Amplifier™ utilizes a common technique used in western blotting to overcome the typical problems that occur in IHC, mainly uneven staining, lack of reproducibility, and high antibody costs. The introduction of the patent-pending Antibody Amplifier™ and Antibody Amplifier Eclipse™ have revolutionized IHC by combining techniques used in western blotting and applying them to IHC.

 

Video Demo Protocols

 


Anti-p53 at 1:1,000,000 dilution on mouse colon paraffin section

Anti-p53 at 1:1,000,000 dilution on mouse colon paraffin section

Anti-Ki67 at 1:1,000,000 dilution on mouse colon paraffin section

 


 

How to Order

  • Use the "Add to Cart" button next to each product for online ordering or contact us at sales@ihcworld.com for purchase order or inquires. Please indicate the product name, catalog# and quantity.

Antibody Amplifier                                 

Catalog No.
IW-AA1
Unit:
Each
Price:  $75
Description: One unit is one Antibody Amplifier consisting of 12 compartments, each fitting one standard microscope slide.

The Antibody Amplifier™ utilizes a common technique used in western blotting and applies it to IHC. During antibody incubation, slides are submerged and rocked in a greatly diluted antibody solution. Antibody dilutions at 1:1,000,000 are frequently seen using the Antibody Amplifier™.


Antibody Amplifier Eclipse                     

Catalog No.: IW-AA2

Unit: Each
Price:  $85

Description: One unit is one Antibody Amplifier consisting of 12 compartments, each fitting one standard microscope slide.

 


The Antibody Amplifier Eclipse offers all the benefits of the Antibody Amplifier plus the security of super opaque construction for all your fluorescence needs.The Antibody Amplifier Eclipse™ even improves the ease and confidence of live cell experiments.

Amplifying Antibody Dilution Buffer     

Catalog No. IW-AA3
Unit: 500 ml
Price:
$95

This buffer can be used to dilute both primary and secondary antibodies during immunohistochemistry and immunofluorescence staining. There is no preservatives to interfere with key enzymes, such as peroxidase. Each bottle of Amplifying Antibody Dilution Buffer is enough for 160 slides.


Amplifying Wash Buffer 20X                      

Catalog No. IW-AA4
Unit: 500 ml
Price: $98

This TBST-based buffer consistently and gently washes your slides, and is high quality, low cost wash buffer for all your IHC and IF needs.

Epitope Unmasking Solution 10X           

Catalog No. IW-AA5
Unit: 500 ml
Price: $195

A citric acid based solution designed to restore epitope structure and enhance antigen-antibody recognition in IHC and IF. This solution reverts cross links formed during fixation, and restores epitope configuration. The Epitope Unmasking Solution will enhance the binding of your primary antibody, and result in a strong signal with little background.

Antibody Amplifier Kit 1                         

 

Catalog No. IW-AAK-1

Unit: Kit

Price: $198

 

 


This Kit offers the classic Antibody Amplifier, one bottle of Amplifying Antibody Dilution Buffer, and one bottle of Amplifying IHC Wash Buffer.

Each bottle of Amplifying Antibody Dilution Buffer is enough for 160 samples, and comes ready-to use. The Amplifying IHC Wash Buffer is 20X concentrated to accomodate the most prolific experiments.

Antibody Amplifier Eclipse Kit 2                




Catalog No. IW-AAK-2

Unit: Kit

Price: $215

 

The Antibody Amplifier Eclipse is perfect for those investigators new to immunofluorescence, or those in need of better, more consistent results. Each kit contains one ultra-opaque Antibody Amplifier Eclipse, one bottle of Amplifying Antibody Dilution Buffer and one bottle of Amplifying IHC Wash Buffer.

Each bottle of Amplifying Antibody
Dilution Buffer is enough for 160 samples, and comes ready-to use. The Amplifying IHC Wash Buffer is 20X concentrated to accomodate the most prolific experiments.

Antibody Amplifier/Eclipse Kit 3            


Catalog No.
IW-AAk-3
Unit: Kit
Price:
$285
Description:
One classic Antibody Amplifier, + Antibody Amplifier Eclipse +1 bottle of Amplifying Antibody Dilution Buffer + 1 bottle of Amplifying IHC Wash Buffer.

This is the ultimate IHC and immunofuorescence kit. It contains both the Antibody Amplifier and the Antibody Amplifier Eclipse, plus one bottle of Amplifying Antibody Dilution Buffer and one bottle of Amplifying IHC Wash Buffer. This kit gives your lab many options, from IHC to Immunofluorescence to even live cell experiments.

Each bottle of Amplifying Antibody Dilution Buffer is enough for 160 samples, and comes ready-to use. The
Amplifying IHC Wash Buffer is 20X concentrated to accomodate the most prolific experiments.

Super Amplifying IHC Kit with Antibody Amplifier


Catalog No. IW-AAk-4
Unit: Kit
Price:
$265

The Super Amplifying Kit offers the classic Antibody Amplifier™, one 500ml bottle of Amplifying Antibody Dilution Buffer™, one 500ml bottle of 10X Amplifying IHC Wash Buffer™ and one 250ml bottle of 10X Epitope Unmasking Solution.

Super Amplifying IHC Kit with Antibody Amplifier Eclipse


Catalog No. IW-AAk-5
Unit: Kit
Price:
$275

The Super Amplifying IF Kit offers the Antibody Amplifier Eclipse™, one 500ml bottle of Amplifying Antibody Dilution Buffer ™, one 500ml bottle of 20X Amplifying IHC Wash Buffer™, and one 250ml bottle of 10X Epitope Unmasking Solution.

Super Amplifying IHC Kit with Antibody Amplifier and Antibody Amplifier Eclipse


Catalog No. IW-AAk-6
Unit: Kit
Price:
$325

The Super Amplifying IHC/IF Kit offers the classic Antibody Amplifier™, Antibody Amplifier Eclipse™, one 500ml bottle of Amplifying Antibody Dilution Buffer ™, one 500ml bottle of 20X Amplifying IHC Wash Buffer™, and one 250ml bottle of 10X Epitope Unmasking Solution.

Ultra Amplifying IHC Kit for the Detection of Cell Stress with the Antibody Amplifier- 150 slides



Catalog No. IW-AAk-7
Unit: Kit
Price:
$525

The Ultra Amplifying IHC Kit for the Detection of Cell Stress (Amplifier version) contains one classic Antibody Amplifier, one 500ml bottle of Amplifying Dilution Buffer, one 500ml bottle of 20X Amplifying Wash Buffer, and one 250ml bottle of 10X Epitope Unmasking Solution. This cell stress focus kit also includes four antibodies which are important indicators of cellular stress response: anti-DNA Damage, anti-Nitrotyrosine, anti-p53, and anti-p53-phospho-serine 15. These antibodies have been vigorously tested using the ProHisto system. This kit provides enough reagents for processing 150 slides.

Ultra Amplifying IF Kit for the Detection of Cell Stress with the Antibody Amplifier Eclipse- 150 slides


Catalog No. IW-AAk-8
Unit: Kit
Price:
$545


The Ultra Amplifying IF Kit for the detection of Cellular Stress (Antibody Amplifier Eclipse version) contains one Antibody Amplifier Eclipse, one 500ml bottle of Amplifying Dilution Buffer, one 500ml bottle of 20X Amplifying Wash Buffer, one 250ml bottle of 10X Epitope Unmasking Solution. This cell stress focus kit also includes four antibodies which are important indicators of cellular stress response: anti-DNA Damage, anti-Nitrotyrosine, anti-p53, and anti-p53-phospho-serine 15. This kit includes enough reagents for 150 slides.

Ultra Amplifying IHC Kit for the Detection of Cell Stress with the Antibody Amplifier-300 slides


Catalog No. IW-AAk-9
Unit: Kit
Price:
$995


The Ultra Amplifying IHC Kit for the detection of Cellular Stress (Antibody Amplifier version) contains one classic Antibody Amplifier, one 500ml bottle of Amplifying Dilution Buffer, one 500ml bottle of 20X Amplifying Wash Buffer, and one 500ml bottle of 10X Epitope Unmasking Solution. This cell stress focus kit includes four antibodies which are important indicators of cellular stress response: anti-DNA Damage, anti-Nitrotyrosine, anti-p53, and anti-p53-phospho-serine 15. This kit includes enough reagents to process 300 slides.

Ultra Amplifying IF Kit for the Detection of Cell Stress with the Antibody Amplifier Eclipse -300 slides


Catalog No. IW-AAk-10
Unit: Kit
Price:
$998


The Ultra Amplifying IF Kit for the detection of Cellular Stress (Antibody Amplifier Eclipse version) contains one Antibody Amplifier Eclipse, one 500ml bottle of Amplifying Dilution Buffer, one 500ml bottle of 20X Amplifying Wash Buffer, and one 500ml bottle of 10X Epitope Unmasking Solution. This cell stress focus kit also includes four antibodies which are important indicators of cellular stress response: anti-DNA Damage, anti-Nitrotyrosine, anti-p53, and anti-p53-phospho-serine 15. This kit provides enough reagents for processing 300 slides.

 

Primary Antibodies

These antibodies have been lot tested, in order to guarantee they work well. So the actual antibody you buy has already been optimized for the Antibody Amplifier method.

Catalog# Name Description Price
IW-PA10 Calnexin-CT antibody Rabbit anti-Calnexin-CT polyclonal 
antibody
$130
IW-PA70 Hsp90 antibody Mouse anti-Hsp90 monoclonal antibody  $136
IW-PA20 CamKII antibody Mouse anti-CamKII monoclonal antibody  $93
IW-PA80 Nitrotyrosine antibody Mouse anti-nitrotyrosine monoclonal 
antibody
$126
IW-PA30 DNA/RNA Damage antibody Mouse monoclonal anti- DNA/RNA 
oxidative damage antibody
$118
IW-PA90 p38 MAPKinase antibody Mouse anti-p38 monoclonal antibody  $109
IW-PA40 DNMT1 antibody Mouse anti-DNA methyltransferase 1 monoclonal antibody $215
IW-PA100 PUMA antibody Rabbit anti-PUMA (N-terminal) 
polyclonal antibody
$121
IW-PA50 ERK 1/2 antibody Rabbit anti-ERK1/2 polyclonal 
antibody
$115
IW-PA110 Ubiquitin antibody Rabbit anti-Ubiquitin polyclonal 
antibody
$138
IW-PA60 Hsp70 antibody Rabbit anti-Hsp70 polyclonal antibody  $135

 


 

How to Order
  • Use the "Add to Cart" button next to each product for online ordering or contact us at sales@ihcworld.com for purchase order or inquires. Please indicate the product name, catalog# and quantity.

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