Reagent and Dye Solubility Chart

 

Reagent and Dye Solubility Chart

 

Roy C. Ellis

IMVS Division of Pathology

The Queen Elizabeth Hospital

Woodville Road, Woodville, South Australia 5011

Email: roy.ellis@imvs.sa.gov.au

 

 

 

This is to be used as a guide only as solubility data varies between manufacturers for the same product especially for dyes)1-3

COMPOUND

SOLUBILITY in 100 ml WATER (g)

SOLUBILITY in 100 ml ALCOHOL (g)

1,4-naphthoqinone

slight

 

1-naphthol

very slightly

very

2-butoxyethanol

soluble

soluble

2-ethoxyethanol

miscible

 

2-mercaptoethanol

miscible

miscible

2-nitropropane

1.7 ml

very

4-dimethylaminobenzaldehyde

slight

 

5-bromo-4-chloro-3-indolyl

phosphate

insoluble

soluble

Absolute ethanol

miscible

 

Acacia

insoluble

 

Acetic acid

miscible

 

Acetone

miscible

 

Acid fuchsin

20

0.25

Acridine orange

very

soluble

Acriflavine

very

 

Adenosine 5-triphosphate

soluble

 

Agar

insoluble (cold)

 

slowly soluble (hot)

 

Agarose

soluble

 

Alcian blue

5

1.6

Alizarin red S

7.7

0.15

Aluminium ammonium sulphate

5

insoluble

Aluminium chloride

70

100

Aluminium oxide

insoluble

insoluble

Aluminium potassium sulphate

5 (cold)

 

140 (hot)

insoluble

Aluminium sulphate

28

insoluble

Ammonium carbonate

Ammonium chloride

37.4

soluble

Ammonium ferrous sulphate

soluble

insoluble

Ammonium hydroxide

Ammonium nitrate

soluble

 

Ammonium oxalate

soluble

 

Ammonium sulphate

slight

 

Ammonium sulphide

miscible

 

Aniline blue

50

insoluble

Arsenic trioxide

2.1

soluble

Auramine O

0.7

4.5

Azophloxine

3

slight

Azur A

soluble

soluble

Azur B

soluble

soluble

Basic fuchsin

0.4

8

Benzoyl peroxide

less than 1%

slightly

Biebrich scarlet

soluble

0.05

Bismark brown

1.36

1.1

Bromine

slight

 

Calcium chloride

soluble

soluble

Calcium hypochlorite

soluble

 

Carbazole

insoluble

 

Carbon tetrachloride

0.05 ml

miscible

Carmine

soluble

slight

Carminic acid

8.3

 

Celestin blue

2

1.5

Cellosolve

miscible

miscible

Cetyl alcohol

insoluble

 

Chloral hydrate

387.1

 

Chloroform

0.8

miscible

Chromic potassium sulphate

soluble

 

Chromium oxide

Chromium trioxide

very

 

Chromotrop 2R

19.3

0.17

Citric acid

207.5

 

Clearene

insoluble

 

Cobalt chloride

Congo red

soluble

0.05

Copper acetate

7.2

 

Copper sulphate

very

slightly

Cresyl fast violet

0.38

0.75

Crystal ponceau 6R

0.8

0.05

Crystal violet

1.08

13.87

Cupronilic blue

Cyanuric chloride

D-glucose

very

 

Dextran sulphate

very

 

Dextrin

very

 

Di-N-butyl phthalate

0.04

miscible

Di-potassium orthophosphate

very

slight

Di-sodium hydrogen

orthophosphate

soluble

insoluble

Diaminobenzidine

Dichloroethane

1.132

 

Diethyl ether

8.4%

miscible

Dimethyl formamide

Dimethyl sulphoxide

very

 

Diphenylamine

0.03

very

Dithiol

insoluble

 

Eosin B

39.11

0.75

Eosin Y

44.2

2.2

Epoxypropane

very

 

Erythrosin

11.1

1.87

Ethyl maleamide

Ethylene

Diaminetetraaceticacid

slight

 

Ethylene glycol

soluble

miscible

Fast blue b salt

very

 

Fast garnet GBC

5

 

Fast green FCF

16

0.35

Fast ponceau

soluble

1.5

Fast red B

20

 

Fast red TR

20

 

Ferric chloride

very

 

Ferric sulphate

slight

 

Fluorescein

0.08

2.21

Formaldehyde

very

 

Formamide

soluble

soluble

Formic acid

miscible

miscible

Gallocyanin

insoluble

slight

Giemsa

very

 

Glutaraldehyde

very

very

Glycerol

soluble

 

Glycerol monostearate

insoluble

 

Glycine

soluble

insoluble

Glycol methacrylate

very

 

Gold chloride

very

 

Haematin

slight

 

Haematoxylin

1.5

very

Hydrochloric acid

very

miscible

Hydrogen peroxide

very

 

Hydroquinone

7.2

 

Imidazole

very

 

Indigo carmine

1.1

 

Iodine

0.03

 

Iron alum

soluble

insoluble

Iron oxide

Iso-amyl alcohol

2.4%

miscible

Isopentane

insoluble

 

Isopropanol

Janus green B

5.2

1.1

l-lysine monohydrochloride

very

 

L-lysine monohydrochloride

soluble

 

Lead acetate

soluble

 

Lead nitrate

58.9

 

Levamisole

Light green SF

20.35

0.82

Lithium carbonate

slight

 

Luxol fast blue

slight

soluble

Magnesium chloride

166.3

 

Magnesium oxide

Magnesium sulphate

123.1

 

Malachite green

7.6

7.52

Manganese chloride

72.3

 

Martius yellow

0.5

1.9

Mercaptoethanol

Mercuric chloride

Mercuric oxide

Metanil yellow

5.36

1.45

Methanol

miscible

miscible

Methenamine

Methyl acetate

2.45

soluble

Methyl blue

soluble

insoluble

Methyl green

1.5

0.1

Methyl methacrylate

slight

 

Methyl violet

3

13.2

Methylamine

very

soluble

Methylene blue

3.55

1.5

Mollifex

soluble

 

N,N-D-methyl-p-

phenylenediamine

soluble

N-methylaniline

slight

soluble

Naphthol

slight

very

Naphthol AS-D chloroacetate

soluble

Naphthylamine

slight

 

Neutral red

5.64

2.45

New fuchsin

1.13

3.26

Nickel sulphate

Nile blue sulphate

0.16

0.62

Nitric acid

soluble

 

Nitro blue tetrazolium

slightly

 

Nuclear fast red

0.25

 

Oil red O

slight

0.5

Orange G

10.36

0.22

Orcein

insoluble

 

Osmium tetroxide

Oxalic acid

100

40

Paraformaldehyde

slight

insoluble

Pararosaniline chloride

0.26

5.93

Patent blue

8.4

5.23

Perchloric acid

very

 

Periodic acid

very

 

Phenazine methosulphate

 

Phenol

7

very

Phenylenediamine

moderate

 

Phenylhydrazine

slight

very

Phenylhydrazinium chloride

very

soluble

Phloxine

51

9

Phosphine 3R

soluble

soluble

Phosphomolybdic acid

293.5

 

Phosphotungstic acid

250

 

Picric acid

1.2

9

Poly-l-lysine

Polyethylene glycol

soluble

soluble

Polyvinyl alcohol

Polyvinylpyrrolidone

very

 

Ponceau 6R

13

0.01

Potassium acetate

219.2

 

Potassium cacodylate

soluble

insoluble

Potassium carbonate

soluble

insoluble

Potassium chlorate

soluble

soluble

Potassium chloride

35.95

 

Potassium citrate

Potassium dichromate

15

 

Potassium dihydrogen

orthophosphate

33 at 0°C

 

83.5 at 20°C

insoluble

Potassium ferricyanide

47.15

 

Potassium ferrocyanide

31.6

 

Potassium hydroxide

10

soluble

Potassium iodate

Potassium iodide

149.34

 

Potassium metabisulphite

moderate

 

Potassium nitrate

38.8

 

Potassium permanganate

6.4

very

Pyridine

Pyrocatechol

Pyronin Y

9

0.6

Quinacrine

3 (cold)

slight

Resorcin

soluble

soluble

Rhodamine B

0.8

1.5

Rosanilin

0.4

8.16

Rubeanic acid

slight

soluble

Safanin O

5.5

3.5

Safsolv

insoluble

very

Salicylic acid

slight

soluble

Silicon carbide

Silver nitrate

250.4

 

Sirius red

5

2.35

Sodium acetate

50.6

 

Sodium arsenate

very

 

Sodium azide

decomposes

slight

Sodium bicarbonate

9

 

Sodium borate

5.5

 

Sodium borohydride

liberates hydrogen

 

Sodium cacodylate

Sodium carbonate

7.1 at 0°C

 

22 at 22°C

insoluble

Sodium chloride

soluble

slight

Sodium chromate

87.3

0.34

Sodium citrate

Sodium dihydrogen

orthophosphate

60 at 0°C

 

427 at 100°C

insoluble

Sodium dithionite

very

 

Sodium hydrosulphite

very

soluble

Sodium hydroxide

42 at 0°C

 

347 at 100°C

soluble

Sodium hypochlorite

soluble

 

Sodium iodate

9.35

 

Sodium lactate

very

 

Sodium metabisulphite

Sodium nitrate

very

 

Sodium nitrite

67

 

Sodium periodate

14.4

 

Sodium phosphate

soluble

 

Sodium pyrophosphate

6.7

insoluble

Sodium salicylate

Sodium sulphate

29.8

 

Sodium sulphite

31.3

 

Sodium thiocyanate

very

 

Sodium thiosulphate

202.1

 

Sodium tungstate

soluble

 

Solochrome cyanin

soluble soluble

 

Stannous chloride

Sucrose

211.4

slight

Sudab black B

insoluble

1.13

Sudan III

insoluble

0.19

Sudan IV

insoluble

0.1

Sulphosalicylic acid

Sulphuric acid

very

soluble

Tannic acid

very

 

Tartrazine

11

0.1

Thioflavine T

2.1

3.1

Thionin

0.25

0.25

Thionyl chloride

Thymol

0.08

100

Toluene

0.05

soluble

Toluidine blue

3.8

0.6

Tri-sodium citrate

72

 

Tri-sodium orthophosphate

28

insoluble

Tri-sodium phosphate

2.8

insoluble

Trichloracetic acid

1205.5

 

Trichloroethane

slight

soluble

Trimethylamineformic acid

 

Tris

soluble

 

Uranyl nitrate

soluble

soluble

Urea

1000

very

Victoria blue R

0.5

4

Zinc chloride

soluble

 

Zinc sulphate

 

 

 

References:


1. Ajax Chemicals. Material Safety Data Sheets. Ajax Chemicals Pty Ltd, Auburn, New South Wales, Australia. 1994
 

2. Bancroft JD and Stevens A. Theory and Practice of Histological Techniques, 3rd edition. London. Churchill Livingstone 1990
 

3. Lillie RD. H.J. Conn's Biological Stains, 9th edition. Baltimore. The Williams and Wilkins Co. 1977